Products: Primary Antibodies

Anti-Kv1.5 K+ channel (K7/45)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-011	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-011	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IP WB

Immunogen:

Fusion protein amino acids 542-602 (cytoplasmic C-terminus) of rat Kv1.5 (accession number P19024)

Human: 83% identity (51/61 amino acids)
Mouse: 96% identity (59/61 amino acids)

Cross Reactivity:

None Reported

Protein Name(s):

Potassium voltage-gated channel subfamily A member 5 (RCK7) (RK4) (Voltage-gated potassium channel subunit Kv1.5)

Gene Name(s):

Kcna5

Antibody Registry ID:

Purified: AB_2131324
TC Supernatant: AB_10675288

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

70 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Nieves-Cintrón M, Nystoriak MA, Prada MP, Johnson K, Fayer W, Dell'Acqua ML, Scott JD, Navedo MF.. (2015), 'Selective down-regulation of KV2.1 function contributes to enhanced arterial tone during diabetes..' The Journal of Biological Chemistry. 10.1074/jbc.M114.622811.
El Gebeily G, El Khoury N, Mathieu S, Brouillette J, Fiset C.. (2015), 'Estrogen regulation of the transient outward K(+) current involves estrogen receptor α in mouse heart..' Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2015.07.013.
Dufour MA, Woodhouse A, Goaillard JM.. (2015), 'Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development..' Journal of Neuroscience Research. 10.1002/jnr.23382.
Speca DJ, Ogata G, Mandikian D, Bishop HI, Wiler SW, Eum K, Wenzel HJ, Doisy ET, Matt L, Campi KL, Golub MS, Nerbonne JM, Hell JW, Trainor BC, Sack JT, Schwartzkroin PA, Trimmer JS.. (2014), 'Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability..' Genes, Brain and Behavior. 10.1111/gbb.12120.
Lopez-Izquierdo A, Pereira RO, Wende AR, Punske BB, Abel ED, Tristani-Firouzi M.. (2014), 'The absence of insulin signaling in the heart induces changes in potassium channel expression and ventricular repolarization..' American Journal of Physiology-Heart and Circulatory Physiology. 10.1152/ajpheart.00849.2013.
Ovsepian SV, Steuber V, Le Berre M, O'Hara L, O'Leary VB, Dolly JO.. (2013), 'A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets..' The Journal of Physiology. 10.1113/jphysiol.2012.249706.
Yamada J, Jinno S.. (2013), 'Novel objective classification of reactive microglia following hypoglossal axotomy using hierarchical cluster analysis. .' Journal of Comparative Neurology. 10.1002/cne.23228.
Fulton S, Thibault D, Mendez JA, Lahaie N, Tirotta E, Borrelli E, Bouvier M, Tempel BL, Trudeau LE.. (2011), 'Contribution of Kv1.2 voltage-gated potassium channel to D2 autoreceptor regulation of axonal dopamine overflow..' The Journal of Biological Chemistry. 10.1074/jbc.M110.153262.
Stockwin LH, Yu SX, Borgel S, Hancock C, Wolfe TL, Phillips LR, Hollingshead MG, Newton DL.. (2010), 'Sodium dichloroacetate selectively targets cells with defects in the mitochondrial ETC. .' International Journal of Cancer . 10.1002/ijc.25499.
Cidad P, Moreno-Domínguez A, Novensá L, Roqué M, Barquín L, Heras M, Pérez-García MT, López-López JR.. (), 'Characterization of ion channels involved in the proliferative response of femoral artery smooth muscle cells. .' Arterioscler Thromb Vasc Biol.. 10.1161/ATVBAHA.110.205187.
Tobin AA, Joseph BK, Al-Kindi HN, Albarwani S, Madden JA, Nemetz LT, Rusch NJ, Rhee SW.. (2009), 'Loss of cerebrovascular Shaker-type K(+) channels: a shared vasodilator defect of genetic and renal hypertensive rats. .' American Journal of Physiology-Heart and Circulatory Physiology. 10.1152/ajpheart.00991.2008.
Miller MN, Okaty BW, Nelson SB.. (2008), 'Region-specific spike-frequency acceleration in layer 5 pyramidal neurons mediated by Kv1 subunits. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2940-08.2008.
Nanduri J, Wang N, Bergson P, Yuan G, Ficker E, Prabhakar NR.. (2008), 'Mitochondrial reactive oxygen species mediate hypoxic down-regulation of hERG channel protein. .' Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2008.06.028.
Pott C, Ren X, Tran DX, Yang MJ, Henderson S, Jordan MC, Roos KP, Garfinkel A, Philipson KD, Goldhaber JI.. (2007), 'Mechanism of shortened action potential duration in Na+-Ca2+ exchanger knockout mice. .' American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00177.2006.
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Dufour MA, Woodhouse A, Goaillard JM.. (2014), 'Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development. .' Journal of Neuroscience Research. 10.1002/jnr.23382.
Ovsepian SV, Steuber V, Le Berre M, O'Hara L, O'Leary VB, Dolly JO.. (2013), 'A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets. .' The Journal of Physiology. 10.1113/jphysiol.2012.249706.
Irani SR, Pettingill P, Kleopa KA, Schiza N, Waters P, Mazia C, Zuliani L, Watanabe O, Lang B, Buckley C, Vincent A.. (2012), 'Morvan syndrome: clinical and serological observations in 29 cases. .' Annals of Neurology. 10.1002/ana.23577.
He S, Shao LR, Rittase WB, Bausch SB.. (), 'Increased Kv1 channel expression may contribute to decreased sIPSC frequency following chronic inhibition of NR2B-containing NMDAR. .' Neuropsychopharmacology. 10.1038/npp.2011.320.
Al-Sabi A, Kaza S, Le Berre M, O'Hara L, Bodeker M, Wang J, Dolly JO.. (2011), 'Position-dependent attenuation by Kv1.6 of N-type inactivation of Kv1.4-containing channels. .' Biochemical Journal. 10.1042/BJ20102169.
Prüss H, Dewes M, Derst C, Fernández-Klett F, Veh RW, Priller J.. (), 'Potassium channel expression in adult murine neural progenitor cells. .' Neuroscience. 10.1016/j.neuroscience.2011.02.021.
Fulton S, Thibault D, Mendez JA, Lahaie N, Tirotta E, Borrelli E, Bouvier M, Tempel BL, Trudeau LE.. (), 'Contribution of Kv1.2 voltage-gated potassium channel to D2 autoreceptor regulation of axonal dopamine overflow. .' The Journal of Biological Chemistry. 10.1074/jbc.M110.153262.
Gazula VR, Strumbos JG, Mei X, Chen H, Rahner C, Kaczmarek LK.. (2010), 'Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons. .' Journal of Comparative Neurology. 10.1002/cne.22393.