Products: Primary Antibodies

Anti-Kir6.1 potassium channel (N366/60)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-394	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-394	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 306-424 (cytoplasmic C-terminus) of rat Kir6.1 (also known as ATPsensitive inward rectifier potassium channel 8, Potassium channel inwardly rectifying subfamily J member 8, uKATP-1 and Kcnj8, accession number Q63664)
Mouse: 99% identity (118/119 amino acids identical)
Human: 96% identity (115/119 amino acids identical)
50% identity with Kir6.2

Cross Reactivity:

Does not cross-react with Kir6.2

Protein Name(s):

ATP-sensitive inward rectifier potassium channel 8 (Inward rectifier K(+) channel Kir6.1) (Potassium channel, inwardly rectifying subfamily J member 8) (uKATP-1)

Gene Name(s):

Kcnj8

Antibody Registry ID:

Purified: AB_2491087
TC Supernatant: AB_2336907

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

45 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.