Products: Primary Antibodies

Anti-Kir3.3 Antibody (N455/15)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-445	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-445	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

IHC WB

Target Description:

Potassium inwardly rectifying channel subfamily J member 9 is encoded by the gene KCNJ9. KCNJ9 is a member of the inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ9 subfamily. KCNJ9 is an integral membrane protein and inward-rectifier type potassium channel, which has a greater tendency to allow potassium to flow into the cell rather than out of the cell. KCNJ9 is controlled by G-proteins and associates with another G-protein-activated potassium channel to form a heteromultimeric pore-forming complex. KCNJ9 is expressed in the brain and skeletal muscle. Diseases associated with KCNJ9 include Pendred Syndrom and Seizures, Sensorineural Deafness, Ataxia, Mental Retardation, and Electrolyte Imbalance.

Immunogen:

Fusion protein amino acids 1-21 and 341-393 (fusion of cytoplasmic N- and C-termini) of mouse Kir3.3 (also known as Inward rectifier K(+) channel Kir3.3, Potassium channel, inwardly rectifying subfamily J member 9, G protein-activated inward rectifier potassium channel 3, Kcnj9 and Girk3, accession number P48543)

Protein Name(s):

Inward rectifier K(+) channel Kir3.3 (Potassium channel, inwardly rectifying subfamily J member 9) (G protein-activated inward rectifier potassium channel 3) (Kcnj9) (Girk3)

Gene Name(s):

Kcnj9 Girk3

Antibody Registry ID:

Purified: AB_2686912
TC Supernatant: AB_2619638

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

40 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.