Products: Primary Antibodies

Anti-KCNU1/Slo3 pH-sensitive maxi-K+ channel Antibody (N2/16)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-056	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-056	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

ICC WB

Target Description:

Potassium calcium-activated channel subfamily U member 1 is encoded by the gene KCNU1. KCNU1 is a member of the potassium channel family, calcium-activated (TC 1.A.1.3) subfamily, KCa5.1/KCNU1 sub-subfamily. KCNU1 is a testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates transport of K+. KCNU1 is expressed in the testis. Diseases associated with KCNU1 include Leukodystrophy, Hypomyelinating, 3 and Coffin-Siris Syndrome 1.

Immunogen:

Fusion protein amino acids 1052-1121 of mouse Slo3 (also known as Potassium channel subfamily U member 1, Pore-forming subunit of the sperm-specific alkalization activated K(+) current, Calcium-activated potassium channel subunit alpha-3, Calcium-activated potassium channel, subfamily M subunit alpha-3, Slowpoke homolog 3, Kcnu1, KCa5, Kcnma3, Kcnmc1 and Ksper, accession number O54982)
50% identity with human and rat KCNU1/Slo3

Protein Name(s):

KCNU1/Slo3 pH-sensitive maxi-K+ channel

Gene Name(s):

Kcnu1 Kcnma3 Ksper Slo3

Antibody Registry ID:

Purified: AB_2131864
TC Supernatant: AB_10698181

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

115 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.