Products: Primary Antibodies

Anti-KCNU1/Slo3 pH-sensitive maxi-K+ channel (N2/16)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-056	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-056	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 1052-1121 of mouse Slo3 (also known as Potassium channel subfamily U member 1, Pore-forming subunit of the sperm-specific alkalization activated K(+) current, Calcium-activated potassium channel subunit alpha-3, Calcium-activated potassium channel, subfamily M subunit alpha-3, Slowpoke homolog 3, Kcnu1, KCa5, Kcnma3, Kcnmc1 and Ksper, accession number O54982) 50% identity with human and rat KCNU1/Slo3

Protein Name(s):

Potassium channel subfamily U member 1 (Calcium-activated potassium channel subunit alpha-3) (Calcium-activated potassium channel, subfamily M subunit alpha-3) (Pore-forming subunit of the sperm-specific alkalization activated K(+) current) (KSper) (Slowpoke homolog 3) (mSlo3) (pH-sensitive maxi potassium channel)

Gene Name(s):

Kcnu1 Kcnma3 Ksper Slo3

Antibody Registry ID:

Purified: AB_2131864
TC Supernatant: AB_10698181

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

115 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Zeng XH, Yang C, Xia XM, Liu M, Lingle CJ.. (2015), 'SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER currents and is critical for normal fertility. .' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1423869112.
Yang C, Zeng XH, Zhou Y, Xia XM, Lingle CJ.. (2011), 'LRRC52 (leucine-rich-repeat-containing protein 52), a testis-specific auxiliary subunit of the alkalization-activated Slo3 channel. .' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1111104108.
Zeng XH, Yang C, Kim ST, Lingle CJ, Xia XM.. (2011), 'Proc Natl Acad Sci U S A..' Proceedings of the National Academy of Science of the United States of America. Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa. .
Wojtovich AP, Sherman TA, Nadtochiy SM, Urciuoli WR, Brookes PS, Nehrke K.. (2010), 'The SLO3 sperm-specific potassium channel plays a vital role in male fertility. .' FEBS Letters. 10.1016/j.febslet.2010.02.005.
Yang CT, Zeng XH, Xia XM, Lingle CJ.. (2009), 'Interactions between beta subunits of the KCNMB family and Slo3: beta4 selectively modulates Slo3 expression and function. .' PLoS One. 10.1371/journal.pone.0006135.
Wang H, Liu J, Cho KH, Ren D.. (2009), 'A novel, single, transmembrane protein CATSPERG is associated with CATSPER1 channel protein. .' Biology of Reproduction . 10.1095/biolreprod.109.077107.
Martínez-López P, Santi CM, Treviño CL, Ocampo-Gutiérrez AY, Acevedo JJ, Alisio A, Salkoff LB, Darszon A.. (2009), 'Mouse sperm K+ currents stimulated by pH and cAMP possibly coded by Slo3 channels. .' Biochemical and Biophysical Research Communications. iochem Biophys Res Commun. 2.