Products: Primary Antibodies

Anti-KChIP2b K+ channel (K60/73)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-004	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-004	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 1-252 (full length) of rat KChIP2b (accession number AAF81756)
Human: 96% identity (244/252 amino acids)
Mouse=100% identity

Cross Reactivity:

No cross-reactivity against rat KChIPs 1,3

Protein Name(s):

Kv channel-interacting protein 2 (KChIP2) (A-type potassium channel modulatory protein 2) (Potassium channel-interacting protein 2)

Gene Name(s):

Kcnip2 Kchip2

Antibody Registry ID:

Purified: AB_2280942
TC Supernatant: AB_10671304

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

35 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

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Cheng CF, Wang WC, Huang CY, Du PH, Yang JH, Tsaur ML. (2016), 'Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons..' Journal of Comparative Neurology. 10.1002/cne.23876.
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El Gebeily G, El Khoury N, Mathieu S, Brouillette J, Fiset C. (2015), 'Estrogen regulation of the transient outward K(+) current involves estrogen receptor α in mouse heart..' Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2015.07.013.
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Rainnie DG, Hazra R, Dabrowska J, Guo JD, Li CC, Dewitt S, Muly EC. (2014), 'Distribution and functional expression of Kv4 family α subunits and associated KChIP β subunits in the bed nucleus of the stria terminalis..' Journal of Comparative Neurology. 10.1002/cne.23435.
Foeger NC, Wang W, Mellor RL, Nerbonne JM. (2013), 'Stabilization of Kv4 protein by the accessory K(+) channel interacting protein 2 (KChIP2) subunit is required for the generation of native myocardial fast transient outward K(+) currents..' The Journal of Physiology. 10.1113/jphysiol.2013.255836.
Özgen N, Lu Z, Boink GJ, Lau DH, Shlapakova IN, Bobkov Y, Danilo P Jr, Cohen IS, Rosen MR. (2012), 'Microtubules and angiotensin II receptors contribute to modulation of repolarization induced by ventricular pacing..' Heart Rhythm. 10.1016/j.hrthm.2012.07.014.
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Pruunsild P, Timmusk T. (2012), 'Subcellular localization and transcription regulatory potency of KCNIP/Calsenilin/DREAM/KChIP proteins in cultured primary cortical neurons do not provide support for their role in CRE-dependent gene expression..' Journal of Neurochemistry. 10.1111/j.1471-4159.2012.07796.x.
Marionneau C, Carrasquillo Y, Norris AJ, Townsend RR, Isom LL, Link AJ, Nerbonne JM. (2012), 'The sodium channel accessory subunit Navβ1 regulates neuronal excitability through modulation of repolarizing voltage-gated K⁺ channels..' Journal of Neuroscience. 10.1523/JNEUROSCI.6450-11.2012.
Suzuki T, Shioya T, Murayama T, Sugihara M, Odagiri F, Nakazato Y, Nishizawa H, Chugun A, Sakurai T, Daida H, Morimoto S, Kurebayashi N. (2012), 'Multistep ion channel remodeling and lethal arrhythmia precede heart failure in a mouse model of inherited dilated cardiomyopathy..' PLoS One. 10.1371/journal.pone.0035353.
Jeyaraj D, Haldar SM, Wan X, McCauley MD, Ripperger JA, Hu K, Lu Y, Eapen BL, Sharma N, Ficker E, Cutler MJ, Gulick J, Sanbe A, Robbins J, Demolombe S, Kondratov RV, Shea SA, Albrecht U, Wehrens XH, Rosenbaum DS, Jain MK. (2012), 'Circadian rhythms govern cardiac repolarization and arrhythmogenesis..' Nature. 10.1038/nature10852.
Marshall GE, Russell JA, Tellez JO, Jhund PS, Currie S, Dempster J, Boyett MR, Kane KA, Rankin AC, Workman AJ. (2011), 'Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade..' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-011-1061-z.
Sun W, Maffie JK, Lin L, Petralia RS, Rudy B, Hoffman DA. (2011), 'DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons..' Neuron. 10.1016/j.neuron.2011.08.008.
Stein AB, Jones TA, Herron TJ, Patel SR, Day SM, Noujaim SF, Milstein ML, Klos M, Furspan PB, Jalife J, Dressler GR. (2011), 'Loss of H3K4 methylation destabilizes gene expression patterns and physiological functions in adult murine cardiomyocytes..' Journal of Clinical Investigation. 10.1172/JCI44641.
Cheng L, Yung A, Covarrubias M, Radice GL. (2011), 'Cortactin is required for N-cadherin regulation of Kv1.5 channel function..' The Journal of Biological Chemistry. 10.1074/jbc.M111.218560.
Bourdeau ML, Laplante I, Laurent CE, Lacaille JC. (2011), 'KChIP1 modulation of Kv4.3-mediated A-type K(+) currents and repetitive firing in hippocampal interneurons..' Neuroscience. 10.1016/j.neuroscience.2010.11.051.
Yang KC, Foeger NC, Marionneau C, Jay PY, McMullen JR, Nerbonne JM. (2010), 'Homeostatic regulation of electrical excitability in physiological cardiac hypertrophy..' The Journal of Physiology. 10.1113/jphysiol.2010.197418.
Norris AJ, Foeger NC, Nerbonne JM. (2010), 'Interdependent roles for accessory KChIP2, KChIP3, and KChIP4 subunits in the generation of Kv4-encoded IA channels in cortical pyramidal neurons..' Journal of Neuroscience. 10.1523/JNEUROSCI.2487-10.2010.
Dabrowska J, Rainnie DG. (2010), 'Expression and distribution of Kv4 potassium channel subunits and potassium channel interacting proteins in subpopulations of interneurons in the basolateral amygdala..' Neuroscience. 10.1016/j.neuroscience.2010.09.011.
Foeger NC, Marionneau C, Nerbonne JM. (2010), 'Co-assembly of Kv4 {alpha} subunits with K+ channel-interacting protein 2 stabilizes protein expression and promotes surface retention of channel complexes..' The Journal of Biological Chemistry. 10.1074/jbc.M110.145185.
Levy DI, Cepaitis E, Wanderling S, Toth PT, Archer SL, Goldstein SA. (2010), 'The membrane protein MiRP3 regulates Kv4.2 channels in a KChIP-dependent manner..' The Journal of Physiology. 10.1113/jphysiol.2010.191395.
Liu W, Deng J, Xu J, Wang H, Yuan M, Liu N, Jiang Y, Liu J. (2010), 'High-mobility group box 1 (HMGB1) downregulates cardiac transient outward potassium current (Ito) through downregulation of Kv4.2 and Kv4.3 channel transcripts and proteins..' Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2010.05.004.
Xu X, Jiang M, Wang Y, Smith T, Baumgarten CM, Wood MA, Tseng GN. (2010), 'Long-term fish oil supplementation induces cardiac electrical remodeling by changing channel protein expression in the rabbit model..' PLoS One. 10.1371/journal.pone.0010140.
Anderson D, Mehaffey WH, Iftinca M, Rehak R, Engbers JD, Hameed S, Zamponi GW, Turner RW. (2010), 'Regulation of neuronal activity by Cav3-Kv4 channel signaling complexes..' Nature Neuroscience. 10.1038/nn.2493.
Wagner M, Rudakova E, Schütz V, Frank M, Ehmke H, Volk T. (2010), 'Larger transient outward K(+) current and shorter action potential duration in Galpha(11) mutant mice..' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-009-0762-z.
Soltysinska E, Olesen SP, Christ T, Wettwer E, Varró A, Grunnet M, Jespersen T. (2009), 'Transmural expression of ion channels and transporters in human nondiseased and end-stage failing hearts..' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-009-0718-3.
Calloe K, Soltysinska E, Jespersen T, Lundby A, Antzelevitch C, Olesen SP, Cordeiro JM. (2010), 'Differential effects of the transient outward K(+) current activator NS5806 in the canine left ventricle..' Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2009.07.017.
Jiang M, Xu X, Wang Y, Toyoda F, Liu XS, Zhang M, Robinson RB, Tseng GN. (2009), 'Dynamic partnership between KCNQ1 and KCNE1 and influence on cardiac IKs current amplitude by KCNE2..' The Journal of Biological Chemistry. 10.1074/jbc.M808262200.
Alexander JC, McDermott CM, Tunur T, Rands V, Stelly C, Karhson D, Bowlby MR, An WF, Sweatt JD, Schrader LA. (2009), 'The role of calsenilin/DREAM/KChIP3 in contextual fear conditioning..' Learning & Memory. 10.1101/lm.1261709.
Monaghan MM, Menegola M, Vacher H, Rhodes KJ, Trimmer JS. (2008), 'Altered expression and localization of hippocampal A-type potassium channel subunits in the pilocarpine-induced model of temporal lobe epilepsy..' Neuroscience. 10.1016/j.neuroscience.2008.07.057.
Marionneau C, Aimond F, Brunet S, Niwa N, Finck B, Kelly DP, Nerbonne JM. (2008), 'PPARalpha-mediated remodeling of repolarizing voltage-gated K+ (Kv) channels in a mouse model of metabolic cardiomyopathy..' Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2008.03.023.
Marionneau C, Brunet S, Flagg TP, Pilgram TK, Demolombe S, Nerbonne JM. (2008), 'Distinct cellular and molecular mechanisms underlie functional remodeling of repolarizing K+ currents with left ventricular hypertrophy..' Circulation Research. 10.1161/CIRCRESAHA.107.170050.
Niwa N, Wang W, Sha Q, Marionneau C, Nerbonne JM. (2008), 'Kv4.3 is not required for the generation of functional Ito,f channels in adult mouse ventricles..' Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2007.10.007.
Menegola M, Trimmer JS. (2006), 'Unanticipated region- and cell-specific downregulation of individual KChIP auxiliary subunit isotypes in Kv4.2 knock-out mouse brain..' Journal of Neuroscience. 10.1523/JNEUROSCI.2783-06.2006.