Products: Primary Antibodies

Anti-JIP-2/IB-2 Antibody (N135/37)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-163	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-163	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Target Description:

Mitogen-activated protein kinase 8 interacting protein 2 is encoded by the gene MAPK8IP2. MAPK8IP2 is a member of the JIP scaffold family. MAPK8IP2 is a scaffold protein that is involved in the regulation of the c-Jun amino-terminal kinase signaling pathway. MAPK8IP2 has been shown to interact with and regulate the activity of MAPK8/JNK1 and MAP2K7/MKK7 kinases. MAPK8IP2 is expressed in the brain and testis. Diseases associated with MAPK8IP2 include Cervical Verrucous Carcinoma and Isthmus Cancer.

Immunogen:

Fusion protein amino acids 226-421 of mouse JIP-2/IB-2 (also known as C-jun-aminoterminal kinase-interacting protein 2, JNK-interacting protein 2,JNK MAP kinase scaffold protein 2, Islet-brain-2 and Mitogen-activated protein kinase 8-interacting protein 2, accession number Q9ERE9) Rat: 94% identity (127/135 amino acids identical) Human: 86% identity (173/200 amino acids identical) Greater than 50% identity with JIP-1

Cross Reactivity:

Does not react with JIP-1

Protein Name(s):

JIP-2/IB-2

Gene Name(s):

Mapk8ip2 Ib2 Jip2

Antibody Registry ID:

Purified: AB_2140888
TC Supernatant: AB_10672307

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

90 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.