Human proNGF Rapid ELISA Assay
The Biosensis proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. Accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately.
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
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| Reagent | 1 Plate Kit | 2 Plate Kit |
|---|---|---|
| proNGF antibody coated 96 well microplate | 12 × 8 wells | 24 × 8 wells | Assay diluent A (1X) | 2 × 25 mL | 4 × 25 mL | Heterophilic antibody Blocker BL-003-1000* | 1 Vial | 2 Vials |
| Recombinant human proNGF standard | 2 × 20 ng | 4 × 20 ng | Quality Control (QC) Sample | 2 Vials (see vial label for amount) | 4 Vials (see vial label for amount) |
| Human proNGF detection antibody (100X) | 1 × 110 µL | 2 × 110 µL | Streptavidin-HRP (100X) | 1 × 110 µL | 2 × 110 µL |
| Wash buffer (10X) | 1 × 33 mL | 2 × 33 mL | TMB substrate (1X) | 1 × 11 mL | 2 × 11 mL |
| TMB stop solution (1X) | 1 × 11 mL | 2 × 11 mL | Plate sealer | Supplied |