Human Oligomeric a-Synuclein Rapid ELISA Assay

Name: Human Oligomeric a-Synuclein Rapid ELISA Assay
Brand: Biosensis
SKU: BEK-2246-2P
Price: 787.0 USD
Availability: InStock

The Biosensis Oligomeric Alpha Synuclein Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of Alpha Synuclein Oligomers in less than 4 hours in human serum, plasma (EDTA) and rat brain. This ELISA shows only minimal cross-reactivity with the monomeric form of α-synuclein and beta and gamma synuclein.

Parallelism of six human serum samples, serially diluted.

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide of expected performance. A standard curve should be generated for each assay.

Spike and Recovery of α-synuclein oligomers in Human Serum

Parallelism of six human serum samples, serially diluted.

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Species Reactivity

Human, Rat

Applications

ELISA

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Data Sheet

SKU: BEK-2246-2P

Volume/Size: 2 Plate Kit

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Product Description

The Biosensis Oligomeric Alpha Synuclein Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of Alpha Synuclein Oligomers in less than 4 hours in human serum, plasma (EDTA) and rat brain only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples and rat brain.

This ELISA kit consists of a pre-coated anti-oligomeric α-synuclein capture antibody, a biotinylated anti-oligomeric α-synuclein detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of α-synuclein oligomers present in samples and protein standards. An α-synuclein oligomer positive control (QC sample) is provided to assure consistent assay performance.

This Oligomeric Alpha Synuclein ELISA kit employs a oligomeric alpha synuclein standard made and stabilized according to Almandoz-Gil et al 2018. This ELISA shows only minimal cross-reactivity with the monomeric form of α-synuclein and beta and gamma synuclein .

Scientific Background

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.

Target

Alpha synuclein oligomers

Immunogen

The oligomers were made from recombinant human alpha-synuclein, expressed in E. coli

Immunogen Species

Human

Range

0.78 ng/mL - 50 ng/mL

Sensitivity

Typical limit of detection (LOD) for α-Synuclein oligomers is less than 700 pg/mL, determined as 150% of the blank value.

Specificity

The Oligomeric alpha synuclein ELISA shows less than 5% cross reactivity with ⍺-, β- and γ-synuclein proteins.

Species Reactivity

Human, Rat

Applications

ELISA

Method of Analysis

Colorimetric

Sample Type

Plasma (EDTA), Serum, Tissue Homogenates

Synonyms

Alpha synculein, Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1

UniProt ID

P37840

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months from purchase.

Storage

Store at 2-8°C

Shipping

Shipped on ice packs

Human Oligomeric a-Synuclein Rapid ELISA Assay Protocol
Technical Note 1 - ELISA Validation
Technical Note 2 - ELISA Data Analysis
Calculation of Antigen Amount in Tissues and Lysates

Reagent	1 P	2 P
Oligomeric α-synuclein antibody coated 96 well microplate	12 × 8 wells	24 × 8 wells
Assay diluent A (1X)	2 × 25 mL	4 × 25 mL
Oligomeric α-synuclein standard, Lyophilized	2 × 50 ng	4 × 50 ng
Oligomeric α-synuclein positive control, Lyophilized	2 Vials (see vial label for amount)	4 Vials (see vial label for amount)
α-synuclein detection antibody (100X)	1 × 110 µL	2 × 110 µL
Streptavidin-HRP (100X)	1 × 110 µL	2 × 110 µL
Wash buffer (10X)	1 × 33 mL	2 × 33 mL
TMB substrate (1X)	1 × 11 mL	2 × 11 mL
TMB stop solution (1X)	1 × 11 mL	2 × 11 mL
Plate sealer	Supplied

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Human Oligomeric a-Synuclein Rapid ELISA Assay

SKU: BEK-2246-2P

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