Human mBDNF CE-Marked ELISA Assay
The Biosensis CE Marked BDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the preferential quantification of mature BDNF in less than 3 hours. This kit consists of a pre-coated mouse monoclonal anti-BDNF capture antibody, a biotinylated anti-BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. This kit is suitable to measure mature BDNF in human serum and citrate-treated plasma samples only. The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore recognize the mature as well as the pro-form of BDNF.
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
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| Reagent | 1 Plate Kit, 96 Tests | 2 Plate Kit, 192 Tests |
|---|---|---|
| BDNF Antibody Coated 96 well microwell strips | 1 plate | 2 plates |
| Assay Diluent A* (Ready to Use) | 25 mL x 2 | 25 mL x 4 |
| Recombinant BDNF Standard (Freeze-dried) | 1000 pg x 2 | 1000 pg x 4 |
| BDNF Detection Antibody (Ready to Use) | 12 mL x 1 | 12 mL x 2 |
| Streptavidin-HRP (Ready to Use) | 12 mL x 1 | 12 mL x 2 |
| Wash Buffer, 20X | 25 mL x 1 | 25 mL x 2 |
| TMB Substrate, 1X | 12 mL x 1 | 12 mL x 2 |
| Stop Solution, 1X | 12 mL x 1 | 12 mL x 2 |
| Plate sealer | Supplied | |