Human GDNF Rapid ELISA Assay

Name: Human GDNF Rapid ELISA Assay
Brand: Biosensis
SKU: BEK-2222-2P
Price: 787.0 USD
Availability: InStock

The Biosensis GDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum. This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. The antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF.

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

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Species Reactivity

Human

Applications

ELISA

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Data Sheet

SKU: BEK-2222-2P

Volume/Size: 2 Plate Kit

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$787.00

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Product Description

The Biosensis GDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards.

This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF.

Guinea pig GDNF has been quantified in serum using the Human GDNF Rapid ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as human GDNF equivalents.

This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Scientific Background

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.

Target

Glial cell line-derived neurotrophic factor (GDNF)

Immunogen

Human recombinant GDNF with N-terminal methionine residue.

Immunogen Species

Human

Range

7.8 - 500 pg/mL

Sensitivity

Typical limit of detection (LOD) for rhGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).

Specificity

Human. The antibodies used in this kit detect guinea pig, mouse and rat GDNF. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.

Species Reactivity

Human

Applications

ELISA

Method of Analysis

Colorimetric

Sample Type

Cell Lysates, Culture Supernatant

Synonyms

Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF

UniProt ID

P39905

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months from purchase.

Storage

Store at 2-8°C.

Shipping

Shipped on ice packs

Human GDNF Rapid ELISA Assay Protocol
Technical Note 1 - ELISA Validation
Technical Note 2 - ELISA Data Analysis
Calculation of Antigen Amount in Tissues and Lysates

Reagent	1 Plate Kit	2 Plate Kit
GDNF antibody coated 96 well microplate	12 × 8 wells	24 × 8 wells
Assay diluent A (1X)	2 × 25 mL	4 × 25 mL
Recombinant human GDNF standard	2 × 1000 pg	4 × 1000 pg
Quality Control (QC) Sample	2 Vials (see vial label for amount)	4 Vials (see vial label for amount)
GDNF detection antibody (100X)	1 × 110 µL	2 × 110 µL
Streptavidin-HRP (100X)	1 × 110 µL	2 × 110 µL
Wash buffer (10X)	1 × 33 mL	2 × 33 mL
TMB substrate (1X)	1 × 11 mL	2 × 11 mL
TMB stop solution (1X)	1 × 11 mL	2 × 11 mL
Plate sealer	Supplied

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Human GDNF Rapid ELISA Assay

SKU: BEK-2222-2P

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