Products: Primary Antibodies

Anti-Histone H4-dimethyl-Arg3 Antibody (N309A/21)

from NeuroMab

Product Summary:

This antibody preferentially recognizes a modified sequence from Histone H4.

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-320	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-320	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

DB ELISA

Target Description:

Histone H4 (which is also known by many other names and is recognized by the accession number P62805) is encoded by the gene H4C1 (which is also known by many other names). Histones organize DNA into nucleosomes and are the main structural component of chromatin.

Immunogen:

Synthetic peptide amino acids 2-11 (SG[dimethyl-R-asymmetric]GKGGKGL) of human Histone H4 (accession number P62805)
Mouse: 100% identity (10/10 amino acids identical)
Rat: 100% identity (10/10 amino acids identical)

Cross Reactivity:

Does not cross-react with unmethylated Histone H4

Protein Name(s):

Histone H4

Gene Name(s):

HIST1H4A H4/A H4FA; HIST1H4B H4/I H4FI; HIST1H4C H4/G H4FG; HIST1H4D H4/B H4FB; HIST1H4E H4/J H4FJ; HIST1H4F H4/C H4FC; HIST1H4H H4/H H4FH; HIST1H4I H4/M H4FM; HIST1H4J H4/E H4FE; HIST1H4K H4/D H4FD; HIST1H4L H4/K H4FK; HIST2H4A H4/N H4F2 H4FN HIST2H4; HIST2H4B H4/O H4FO; HIST4H4

Antibody Registry ID:

Purified: AB_2315853
TC Supernatant: AB_2315852

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Citations and References

Satterlee JS1, Beckel-Mitchener A, McAllister K, Procaccini DC, Rutter JL, Tyson FL, Chadwick LH.. (2015), 'Community resources and technologies developed through the NIH Roadmap Epigenomics Program..' Methods Mol Biol.. 10.1007/978-1-4939-1804-1_2.