Hematoxylin, PROBE
This uniquely formulated Probe hematoxylin is an excellent counterstain for in situ DNA hybridization procedure.
Hematoxylin, PROBE
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- After chromogen staining, wash slide 2-3X with de/di water.
- Apply Probe hematoxylin for 2-3 minutes; wash with tap water 5-7X.
- Soak the slides in Immuno Automation buffer (ImmunoBioScience AR-6561), PBS or any buffer with pH 7.4-8 for 2-3 minutes. Hematoxylin changes color from purple red to blue.
- Wash slide 2-3X with de/di water.
- The slides can be mounted with aqueous mounting medium (ImmunoHistoMount (AR-6503) or equivalent) for chromogens like AEC, DAB, Fast red, BCIP/INT, or Super Fast red. DAB and Super Fast can be mounted with Organic mounting mediums like Organo mount / limonene (AR-6504) or equivalent after dehydrating the tissue with alcohols and organic solvents.