Anti-HCN3 Antibody (N141/28)
Our Anti-HCN3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N141/28. It is KO validated, detects mouse and rat HCN3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunoblot versus lysates of total brain from HCN3 knockout (KO) and wild-type (WT) mice probed with N141/28. Data courtesy of Martin Biel (Ludwig-Maximilians Universität München) and reproduced from the Journal of Biological Chemistry (2013 Cao-Ehlker et al, PMID 23382386).
Product Specific References for Applications and Species
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36395691 | 1:500 | Choi, K., et al. 2022. Presynaptic HCN Channel Activity Is Required For The Expression Of Long-Term Potentiation At Lateral Amygdala To Basal Amygdala Synapses. Biochemical And Biophysical Research Communications, 100-107. |
| 22832570 | not listed | Almanza, A., et al. 2012. Molecular identity, ontogeny, and cAMP modulation of the hyperpolarization-activated current in vestibular ganglion neurons.. Journal of Neurophysiology, 2264-2275. |
| 21945537 | 1:300 | Kanyshkova, T., et al. 2012. Differential regulation of HCN channel isoform expression in thalamic neurons of epileptic and non-epileptic rat strains.. Neurobiology of Disease, 450-461. |
| 21938402 | 1:100 | Anderson, W.D., et al. 2011. Properties and functional implications of I (h) in hippocampal area CA3 interneurons.. Pflügers Archiv: European Journal of Physiology, 895-912. |
| 21159967 | 1:1000 | Giesbrecht, C.J., et al. 2010. Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors.. Journal of Neuroscience, 16970-16982. |
| 20220080 | 1:25 | Yi, E., et al. 2010. Dendritic HCN channels shape excitatory postsynaptic potentials at the inner hair cell afferent synapse in the mammalian cochlea.. Journal of Neurophysiology, 2532-2543. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 23382386 | not listed | Cao-Ehlker, X., et al. 2013. Up-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) by specific interaction with K+ channel tetramerization domain-containing protein 3 (KCTD3).. The Journal of Biological Chemistry, 7580-7589. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34429357 | 1:2000 | Schlusche, A.K., et al. 2021. Developmental HCN channelopathy results in decreased neural progenitor proliferation and microcephaly in mice. PNAS: USA, e20093933118. |
| 26459112 | 1:1000 | Heuermann, R.J., et al. 2016. Reduction of thalamic and cortical Ih by deletion of TRIP8b produces a mouse model of human absence epilepsy.. Neurobiology of Disease, 81-92. |
| 23382386 | not listed | Cao-Ehlker, X., et al. 2013. Up-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) by specific interaction with K+ channel tetramerization domain-containing protein 3 (KCTD3).. The Journal of Biological Chemistry, 7580-7589. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 31589884 | 1:200 | Hughes, B.A., et al. 2020. Chronic ethanol exposure alters prelimbic prefrontal cortical Fast-Spiking and Martinotti interneuron function with differential sex specificity in rat brain. Neuropharmacology, 107805. |
| 23821600 | not listed | Stoenica, L., et al. 2013. HCN1 subunits contribute to the kinetics of I(h) in neonatal cortical plate neurons.. Developmental Neurobiology, 785-797. |