Products: Primary Antibodies

Anti-Haspin/GSG2 Antibody (N128A/2)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-161	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-161	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human

Applications:

ICC IP WB

Target Description:

Histone H3 Associated Protein Kinase is encoded by the gene HASPIN. HASPIN is a member of the protein kinase superfamily, Ser/Thr protein kinase family, and the Haspin subfamily. HASPIN is a serine/threonine - protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. HASPIN may act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment, and normal progression through the cell cycle. HASPIN is expressed in the testis, thymus, bone marrow, prostate, intestine, lung, spleen, and lymph node. No diseases were found to be associated with HASPIN.

Immunogen:

Fusion protein amino acids 1-798 (full-length) of human Haspin (also known as serine/threonineprotein kinase haspin, haploid germ cell-specific nuclear protein kinase, germ cell-specific gene 2 protein and GSG2, accession number Q8TF76); Mouse: 61% identity (493/799 amino acids identical)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

Haspin/GSG2

Gene Name(s):

HASPIN GSG2

Antibody Registry ID:

Purified: AB_2114890
TC Supernatant: AB_10672303

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

90 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.