Glutathione Fluorescent Detection Kit
ICT’s Glutathione Fluorescent Detection Kit is designed to quantitatively measure reduced state glutathione (GSH), and oxidized state glutathione (GSSG) present in a variety of samples. Sample types validated include: human whole blood; serum; EDTA and heparin plasma; urine; and isolated erythrocytes. Most cell lysates and tissue homogenates should also be compatible.
Glutathione Fluorescent Detection Kit
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Ships: 1-2 business days
- Use plate layout template to aid in proper sample and standard identification.
- Pipet 50 µL SSA-treated samples, standards, or control into plate wells.
- Pipet 50 µL Sample Diluent into zero wells.
- Add 25 µL Fluorescent GSH Detection Substrate to each well using a repeater pipet.
- Gently tap plate sides to ensure adequate mixing of the reagents.
- Incubate 15 minutes at room temperature.
- Read the fluorescent emission at 510 nm with excitation at 370-410 nm. This data will be used to determine Free GSH concentration.
- Add 25 µL Reaction Mixture to each well using a repeater pipet.
- Gently tap plate sides to ensure adequate mixing of the reagents.
- Incubate 15 minutes at room temperature.
- Read the fluorescence with excitation at 370-410 nm and emission at 510 nm. This data will be used to determine Total GSH concentration.
Kit 9133:
1 black half-area polystyrene 96-round well non-binding surface treated microtiter plate, #269
Glutathione Standard, 250 µM, 100 µL, #6610
Fluorescent GSH Detection Substrate, 2 vials, #6612
Dry DMSO, 4 mL, #6614
Assay Buffer, 1X, 60 mL, #6616
NADPH Concentrate, 300 µL, #6618
Glutathione Reductase Concentrate, 300 µL, #6620
Oxidized Glutathione Control, 300 µL, #6623
Kit Manual
Kit 9134:
5 black half-area polystyrene 96-round well non-binding surface treated microtiter plate, #269
Glutathione Standard, 250 µM, 300 µL, #6611
Fluorescent GSH Detection Substrate, 4 vials, #6613
Dry DMSO, 20 mL, #6615
Assay Buffer, 1X, 200 mL, #6617
NADPH Concentrate, 1.4 mL, #6619
Glutathione Reductase Concentrate, 1.4 mL, #6621
Oxidized Glutathione Control, 300 µL, #6623
Kit Manual
Product Specific References
| 38834354 | Shirakawa, M, et al. 2024. Rapamycin and Starvation Mitigate Indomethacin-Induced Intestinal Damage through Preservation of Lysosomal Vacuolar ATPase Integrity. The Journal of pharmacology and experimental therapeutics, 108-115. |