Products: Primary Antibodies

Anti-GIT1 (N39B/8)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-094	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-094	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1(also known as G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, Cool-associated and tyrosinephosphorylated protein 1 and Cat-1, accession number Q9Z272)
Human: 93% identity (372/396 amino acids identical)
Mouse: 97% identity (385/396 amino acids identical)
~50% identity with GIT2

Cross Reactivity:

No cross-reactivity against GIT2

Protein Name(s):

ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1)

Gene Name(s):

Git1

Antibody Registry ID:

Purified: AB_2109991
TC Supernatant: AB_10672300

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

90 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Brimer N, Wade R, Vande Pol S.. (2014), 'Interactions between E6, FAK, and GIT1 at paxillin LD4 are necessary for transformation by bovine papillomavirus 1 E6. .' J Virol.. 10.1128/JVI.00552-14.
Wade R, Brimer N, Lyons C, Vande Pol S.. (2011), 'Paxillin enables attachment-independent tyrosine phosphorylation of focal adhesion kinase and transformation by RAS. .' The Journal of Biological Chemistry. 10.1074/jbc.M111.294504.
Won H, Mah W, Kim E, Kim JW, Hahm EK, Kim MH, Cho S, Kim J, Jang H, Cho SC, Kim BN, Shin MS, Seo J, Jeong J, Choi SY, Kim D, Kang C, Kim E.. (), 'GIT1 is associated with ADHD in humans and ADHD-like behaviors in mice. .' Nature Medicine. 10.1038/nm.2330.
Moreau MM, Piguel N, Papouin T, Koehl M, Durand CM, Rubio ME, Loll F, Richard EM, Mazzocco C, Racca C, Oliet SH, Abrous DN, Montcouquiol M, Sans N.. (2010), 'The planar polarity protein Scribble1 is essential for neuronal plasticity and brain function. .' Journal of Neuroscience. 10.1523/JNEUROSCI.6007-09.2010.
Morimura S, Suzuki K, Takahashi K.. (2009), 'BetaPIX and GIT1 regulate HGF-induced lamellipodia formation and WAVE2 transport. .' Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2009.03.083.
Klemmer P, Smit AB, Li KW.. (), 'Proteomics analysis of immuno-precipitated synaptic protein complexes. .' Journal of Proteomics. 10.1016/j.jprot.2008.10.005.