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Products: Primary Antibodies

Anti-GIT1 Antibody (N39B/8)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-094
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-094
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Target Description:

G-protein coupled receptor kinase interacting ArfGAP 1 is encoded by the gene GIT1. GIT1 is a member of the GIT protein family. GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. GIT1 is expressed in numerous tissues with the highest levels in the testis. Diseases associated with GIT1 include Attention Deficit-Hyperactivity Disorder.

Immunogen:

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1(also known as G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, Cool-associated and tyrosinephosphorylated protein 1 and Cat-1, accession number Q9Z272); Human: 93% identity (372/396 amino acids identical); Mouse: 97% identity (385/396 amino acids identical) ; ~50% identity with GIT2

Cross Reactivity:

No cross-reactivity against GIT2

Protein Name(s):

GIT1

Gene Name(s):

Git1

Antibody Registry ID:

Purified: AB_2109991
TC Supernatant: AB_10672300


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

90 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.