Products: Primary Antibodies

Anti-GIT1 Antibody (N39B/8)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-094	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-094	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Target Description:

G-protein coupled receptor kinase interacting ArfGAP 1 is encoded by the gene GIT1. GIT1 is a member of the GIT protein family. GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. GIT1 is expressed in numerous tissues with the highest levels in the testis. Diseases associated with GIT1 include Attention Deficit-Hyperactivity Disorder.

Immunogen:

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1(also known as G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, Cool-associated and tyrosinephosphorylated protein 1 and Cat-1, accession number Q9Z272); Human: 93% identity (372/396 amino acids identical); Mouse: 97% identity (385/396 amino acids identical) ; ~50% identity with GIT2

Cross Reactivity:

No cross-reactivity against GIT2

Protein Name(s):

GIT1

Gene Name(s):

Git1

Antibody Registry ID:

Purified: AB_2109991
TC Supernatant: AB_10672300

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

90 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.