Anti-GFP Antibody (N86/8)

Name: Anti-GFP Antibody
Brand: NeuroMab
SKU: 75-131
Price: 394.0 USD
Availability: InStock

Our mouse GFP monoclonal antibody from NeuroMab is produced in-house from clone N86/8. It detects n/a GFP and is purified by Protein A chromatography. Works in WB, IHC, IP, ELISA, ChIP.

Transfected cell immunofluorescence: COS cells expressing GFP. Blue = Hoechst nuclear stain, Green = GFP, Red = N86/8.

Transfected cell immunoblot extracts of COS cells transfected with GFP; GFP-tagged HCN2, FGF11/FHF3 FGF13/FHF2 and BKBeta2; or untagged Kv2.1 plasmid and probed with N86/8 TC supe.

Transfected cell immunoblot: extracts of COS cells transfected with GFP-tagged MHC, CFP-tagged MHC, YFP-tagged Histone H3.3 or untagged Kv2.1 plasmid and probed with N86/8 (left) or N52A/42 (right) TC supe.

Human skin fibroblasts from healthy individuals were transduced with lentivirus carrying pHAGE-TID1S gene or vector control for 5 days prior to staining vector control with an anti-GFP antibody (Cat. 75-131, 1:200) and TID1S with an anti-TID1L/S antibody. Image from publication CC-BY-4.0. PMID:38495102

Western blot using HEK293T cells lysates expressing EGFP or EGFP-NS1-BP and Flag-p62 K435R(m) treated with sodium arsenite (0.5 mM, 1 h) labeled with anti-Flag (or IgG), anti-GFP (Cat. 75-131, 1:10,000), and anti-HA antibodies. Image from publication, CC-BY-4.0, PMID: 39738171)

Transfected cell immunofluorescence: COS cells expressing GFP. Blue = Hoechst nuclear stain, Green = GFP, Red = N86/8.

Click on image to zoom

Applications

ChIP, ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-131

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GFP

Scientific Background

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N86/8

Isotype

IgG2a

Applications

ChIP, ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

GFP

Molecular Weight

27 kDa

Immunogen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Immunogen Species

Jellyfish

Antibody Registry ID

AB_10671445

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.57

Dilution Ranges

WB: 1:1000
IHC: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Green fluorescent protein

UniProt ID

P42212

Shipping

Shipped on ice packs

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Anti-GFP Antibody (N86/8)

SKU: 75-131

Product Details

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