Products: Primary Antibodies

Anti-GFP (green fluorescent protein) (N86/20)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-413		 Option
Purified		 Volume
100 µL		 Concentration
1 mg/mL		 Price
$275.00
Catalog #
73-413		 Option
TC Supernatant		 Volume
5 mL		 Concentration
Lot dependent		 Price
$275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC WB

Immunogen:

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (also known as GFP, accession number P42212)

Cross Reactivity:

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively) Does not cross-react with Red fluorescent protein (dsRed)

Protein Name(s):

Green fluorescent protein

Gene Name(s):

GFP

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

30 kDa

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.