Products: Primary Antibodies

Anti-Gamma-protocadherin-B2 (N148/30)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-184	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-184	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 719-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-B2 (also known as PCDH-gamma-B2, accession number Q91XX7)
Human: 68% identity (60/88 amino acids identical)
>60% identity with other Gamma-protocadherin-B proteins

Cross Reactivity:

No cross-reactivity against other Gamma-protocadherin-A, -B or -C proteins

Protein Name(s):

Protocadherin gamma B2 (Protocadherin gamma subfamily B, 2)

Gene Name(s):

Pcdhgb2

Antibody Registry ID:

Purified: AB_10675142
TC Supernatant: AB_10672846

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Prasad T, Weiner JA.. (2011), 'Direct and Indirect Regulation of Spinal Cord Ia Afferent Terminal Formation by the γ-Protocadherins. .' Frontiers in Molecular Neuroscience. 10.3389/fnmol.2011.00054.
Lobas MA, Helsper L, Vernon CG, Schreiner D, Zhang Y, Holtzman MJ, Thedens DR, Weiner JA.. (2012), 'Molecular heterogeneity in the choroid plexus epithelium: the 22-member γ-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation. .' Journal of Neurochemistry. 10.1111/j.1471-4159.2011.07587.x.