Human Mouse Rat
ICC IHC WB
Gamma-aminobutyric acid receptor subunit gamma-2, or GABA AR gamma2 is encoded by the gene GABRG2. GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR gamma2 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR gamma2 has been found expressed in stomach and bladder. In brain, GABA AR gamma2 is expressed at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. Mutations in this gene have been associated with epilepsy and febrile seizures. Note that GABA AR ?2 subunit has two forms, designated “short” (?2S) and “long” (?2L) that differ only in eight amino acids inserted into the large intracellular loop of the subunit (Whiting et al., 1990).
Fusion protein amino acids 360-445 (cytoplasmic C-terminus) of human GABA-A-R-Gamma2L (also known as Gamma-aminobutyric acid receptor subunit gamma-2 and GABRG2, accession number P18507-2)
Mouse: 100% identity (86/86 amino acids identical)
Rat: 100% identity (86/86 amino acids identical)90% identity with GABA-A-R-Gamma2S (amino acids 377-384, LLRMFSFK, unique to Gamma2L)
50% identity with GABA-A-R-Gamma1 and GABA-A-R-Gamma3
Does not cross-react with GABA-A-R-Gamma2S, GABA-A-R-Gamma1 or GABAA-A-R-Gamma3
Gamma-aminobutyric acid receptor subunit gamma-2 (GABA(A) receptor subunit gamma-2)
TC Supernatant: AB_2566822
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.