Anti-GABA-A-R-Gamma2L Antibody (N452/69)

Name: Anti-GABA-A-R-Gamma2L Antibody
Brand: NeuroMab
SKU: 75-442
Price: 319.0 USD
Availability: InStock

5 Citations

Our Anti-GABA-A-R-Gamma2L mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N452/69. It detects human, mouse, and rat GABA-A-R-Gamma2L, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat brain membrane immunoblot.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-442

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

GABA-A-R-Gamma2L

Target Description

Gamma-aminobutyric acid receptor subunit gamma-2, or GABA AR gamma2 is encoded by the gene GABRG2. GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR gamma2 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR gamma2 has been found expressed in stomach and bladder. In brain, GABA AR gamma2 is expressed at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. Mutations in this gene have been associated with epilepsy and febrile seizures. Note that GABA AR γ2 subunit has two forms, designated “short” (γ2S) and “long” (γ2L) that differ only in eight amino acids inserted into the large intracellular loop of the subunit (Whiting et al., 1990).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N452/69

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GABRG2

Molecular Weight

45 kDa

Antigen

Fusion protein amino acids 360-445 (cytoplasmic C-terminus) of human GABA-A-R-Gamma2L (accession number P18507-2) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2617122

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
ICC: 1:1000
IP: 4 ug

Conjugate

Unconjugated

Specificity

Does not cross-react with GABA-A-R-Gamma2S, GABA-A-R-Gamma1 or GABAA-A-R-Gamma3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gamma-aminobutyric acid receptor subunit gamma-2 (GABA(A) receptor subunit gamma-2)

UniProt Details

UniProt (Human): P18507
UniProt (Immunogen Species): P18507-2

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunoprecipitation: Mouse
Western Blot: Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
34936876	1:1000	Garcia, J.D., et al. 2021. Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity. Cell Reports, 110142.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
37108794	4ug	Chen, M., et al. 2023. Blue Native PAGE-Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes: Detection of a Cerebellar α1/α6-Subunits Containing γ-Aminobutyric Acid Type A Receptor Subtype. International Journal of Molecular Sciences, 7632.

Western Blot: Mouse
PMID	Dilution	Publication
34936876	1:5000	Garcia, J.D., et al. 2021. Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity. Cell Reports, 110142.
31315046	1:1000	Nathanson, A.J., et al. 2019. Identification of a core amino acid motif within the α subunit of GABAARs that promotes inhibitory synaptogenesis and resilience to seizures. Cell Reports, 670-681.

Western Blot: Rat
PMID	Dilution	Publication
32579917	1:1000	Rajgor, D., et al. 2020. Local miRNA-dependent translational control of GABAAR synthesis during inhibitory long-term potentiation. Cell reports, 31(12), p.107785.. Cell reports, 107785.

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Anti-GABA-A-R-Gamma2L Antibody (N452/69)

SKU: 75-442

Product Details

Product Specific References for Applications and Species

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