Products: Primary Antibodies

Anti-GABA-A-R-Gamma2L Antibody (N452/69)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-442	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-442	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Gamma-aminobutyric acid receptor subunit gamma-2, or GABA AR gamma2 is encoded by the gene GABRG2. GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR gamma2 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR gamma2 has been found expressed in stomach and bladder. In brain, GABA AR gamma2 is expressed at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. Mutations in this gene have been associated with epilepsy and febrile seizures. Note that GABA AR ?2 subunit has two forms, designated “short” (?2S) and “long” (?2L) that differ only in eight amino acids inserted into the large intracellular loop of the subunit (Whiting et al., 1990).

Immunogen:

Fusion protein amino acids 360-445 (cytoplasmic C-terminus) of human GABA-A-R-Gamma2L (also known as Gamma-aminobutyric acid receptor subunit gamma-2 and GABRG2, accession number P18507-2)
Mouse: 100% identity (86/86 amino acids identical)
Rat: 100% identity (86/86 amino acids identical)90% identity with GABA-A-R-Gamma2S (amino acids 377-384, LLRMFSFK, unique to Gamma2L)
50% identity with GABA-A-R-Gamma1 and GABA-A-R-Gamma3

Cross Reactivity:

Does not cross-react with GABA-A-R-Gamma2S, GABA-A-R-Gamma1 or GABAA-A-R-Gamma3

Protein Name(s):

Gamma-aminobutyric acid receptor subunit gamma-2 (GABA(A) receptor subunit gamma-2)

Gene Name(s):

GABRG2

Antibody Registry ID:

Purified: AB_2617122
TC Supernatant: AB_2566822

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

45 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.