Products: Primary Antibodies

Anti-c-FOS Antibody (N486/32)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-506	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-506	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

C-fos belongs the Fos family. Other members include FosB, Fra-1, Fra-2. c-fos binds to JUN/AP1 transcription factor. C-Fos is expressed in placental tissue, fetal liver, developing bone, developing central nervous system, and adult bone marrow. It is expressed in the nucleus and in the endoplasmic reticulum. c-Fos is has a role in signal transduction, cell proliferation, differentiation, and apoptosis. C-Fos is overexpressed in many cancers.

Immunogen:

Fusion protein amino acids 1-377 (full-length) of human FOS (also known as Proto-oncogene c-Fos, G0/G1 switch regulatory protein 7 and G0S7, accession number P01100) Rat: 94% identity (355/377 amino acids identical)
Mouse: 94% identity (353/377 amino acids identical)
<50% identity with FOSL2

Cross Reactivity:

None Reported

Protein Name(s):

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

Gene Name(s):

FOS, G0S7

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

50 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.