Fluoro-Jade B (FJB) Staining Kit

Name: Fluoro-Jade B (FJB) Staining Kit
Brand: Biosensis
SKU: TR-150-FJB
Price: 497.0 USD
Availability: InStock

The Fluoro-Jade B dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.

FJB staining of pilocarpine-treated rat hippocamal CA1 region. Positive cells were abundant in the CA1 of saline-treated SE rats after pilocarpine treatment (A). There were significantly fewer FJB-positive cells in the CA1 as the dose of a protective drug was increased (B, C, D) compared to saline-treated SE rats. Picture courtesy of Gao F et al. (2014), Neuropsychiatr Dis Treat. 10: 2139-2145.

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Species Reactivity

All Mammals, Human, Mouse, Rat

Applications

Flow, ICC

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Data Sheet

SKU: TR-150-FJB

Volume/Size: 30 mg

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Price:

$497.00

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Product Details

Product Description

The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. The fluorescent dye Fluoro-Jade B (FJB), like its more purified brother Fluoro-Jade C (FJC), is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. Fluoro-Jade B differs from FJC in that it is a slightly less refined chemical formulation and thus it does not quite provide the same level of signal to noise or high resolution as FJC. Nonetheless FJB is still widely used and works very well as a marker of degenerating neurons and even glia (see Damjanac M et al., Brain Res. 2007;1128(1):40-9). FJB operates nearly identically in protocol to that of FJC, and Fluoro-Jade B is compatible with several other labeling procedures including immunofluorescent and fluorescent Nissl techniques. Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade B exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.

Note: This product is equivalent to discontinued product AG310 from Merck-Millipore.

Target

Degenerating neurons

Immunogen Species

Species Independent

Specificity

Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.

Species Reactivity

All Mammals, Human, Mouse, Rat

Applications

Flow, ICC

Application Details

Following our detailed protocol, Fluoro-Jade B labels degenerating neurons which are visualized with blue light excitation, while DAPI (not included) counter stains cell nuclei, visualized with ultra-violet illumination. The Fluoro-Jade B dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.

Method of Analysis

Fluorescence

Format

Purified

Physical State

Dry, Coffee brown to brick red powder; hygroscopic powder keep dessicated

Production Notes

Thin layer chromatograpy using cellulose plates and a solvent system of n-propinol, water, and ammonium hydroxide (6:5:2) revealed the presence of two fluorescent isomers and two trace non-fluorescent bands. No amount of fluorescein or Fluoro-Jade was present.

Synonyms

FJB, Fluoro-Jade

Usage Statement

For research use only.

Country of Origin

United States

Expiration

6 months after date of receipt (unopened vial)

Storage

The powdered dye can be stored desiccated at room temperature in the dark. Storage in a desiccator is recommended as FJB is hydroscopic. The 0.01% stock solution will remain stable for 3 months when stored in a refrigerator, in the dark. The 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJB dye solutions are not stable and should not be stored. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions.

Shipping

25°C (ambient)

Fluoro-Jade B Staining Kit Protocol

Materials Provided:

30 mg Fluoro-Jade B, dry powder
Detailed protocol

Equipment and Reagents Required:

Distilled water
ACS grade Ethanol (200 proof) for slide & solution preparation
1% sodium hydroxide in 80% ethanol (basic alcohol solution)
0.1% Acetic Acid solution (in water)
70% ethanol in distilled water
0.06% (KMnO4) potassium permanganate solution
DAPI powder or 100X solution (working range is 0.5-5 µg/mL)
Xylene liquid
Staining dishes/Coplin jars
Cover slips
DPX mounting media or another permanent mounting medium. Non-polar media are preferred over aqueous mounting media such as glycerin/water to obtain high-contrast images (refer to Appendix B in the protocol for a comparative analysis). Traditional fluorescent mounting mediums are not recommended because of their high pH.
Slide warmer
Convection oven

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Fluoro-Jade B (FJB) Staining Kit

SKU: TR-150-FJB

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