Fluoresceinated Goat Anti-Chicken IgY Secondary Antibody

Name: Fluoresceinated Goat Anti-Chicken IgY Secondary Antibody
Brand: Aves Labs
SKU: F-1005
Price: 132.0 USD
Availability: InStock

Our Fluoresceinated Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.

Representative FFPE section of rat brain cerebellum showing Homer1 staining. The sections were stained with anti-Homer1 recombinant chicken Mab (Cat. 78-454) using goat anti-chicken IgY FITC secondary antibody (Cat. F-1005) (green). The stained sections were mounted with Fluoroshield (DAPI & DABCO) mounting medium (Cat. AR-6505). DAPI (blue) stains cell nuclei. Anti-Homer1 stains the postsynaptic densities of the Purkinje cell dendrites in the molecular layer of the cerebellum.

Representative FFPE section of rat brain cerebellum showing Homer1 staining. The sections were stained with anti-Homer1 recombinant chicken Mab (Cat. 78-454) using goat anti-chicken IgY FITC secondary antibody (Cat. F-1005) (green). The stained sections were mounted with Fluoroshield (DAPI & DABCO) mounting medium (Cat. AR-6505). DAPI (blue) stains cell nuclei. Anti-Homer1 stains the postsynaptic densities of the Purkinje cell dendrites in the molecular layer of the cerebellum.

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Species Reactivity

Chicken

Host Species

Goat

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Data Sheet

SKU: F-1005

Volume: 500 µL

Variant

Price:

$132.00

Quantity:

Product Details

Target

Chicken IgY

Format

Affinity Purified

Concentration

1 mg/mL

Clonality

Polyclonal

Isotype

IgG

Host Species

Goat

Immunogen

IgY

Immunogen Species

Chicken

Species Reactivity

Chicken

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

Physical State

Liquid

Production Notes

Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to fluorescein isothiocyanate.

Buffer

Phosphate-buffered saline (PBS) (pH 7.2), bovine serum albumin (0.2%, w/v), sodium azide (0.02%, w/v) (as an anti-microbial agent).

Conjugate

FITC

Specificity

Does not cross-react with mammalian IgGs

Quality Control

Antibodies were analyzed using immunoelectrophoresis (see below) and immunocytochemistry. In this latter test, abundant fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1:10 dilution in PBS), incubation with chicken anti-vimentin antibodies (1:1000 dilution in PBS) and then incubation with GGHL-30F-2 (1:200 dilution in PBS).

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

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Fluoresceinated Goat Anti-Chicken IgY Secondary Antibody

SKU: F-1005

Product Details

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