Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains) and then conjugated with fluorescein isothiocyanate.