Green Fluorescent FAM-Leu-CMK Serine Protease Assay Kit

Quantify intracellular active chymotrypsin-like serine protease activity in vitro. The CMK group alkylates the active site histidine residue. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.



SKU: 950

Size: 100 Tests
Price:
Sale price$576.80
Ships: 1-2 business days
  1. Prepare samples and controls
  2. Dilute cellular wash buffer 1:10 with diH2O
  3. Reconstitute FLISP with 50 µL DMSO
  4. Dilute FLISP 1:5 by adding 200 µL PBS
  5. Add 10 µL FLISP to each sample (~500 µL aliquot of cultured cells)
  6. Incubate ~ 1 hour.
  7. Remove media and wash cells: add wash buffer and spin cells (twice); or add fresh media and incubate 1 hour
  8. If desired, label with additional stains, such as Hoechst, PI, or an antibody
  9. If desired, fix or embed cells
  10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLISP excites at 488 nm and emits at 520 nm.
Kit 949: 25 Tests
  • FAM-Leu-CMK, 1 vial, #6148
  • Hoechst Stain, 1 mL, #639
  • Propidium Iodide, 1 mL, #638
  • 10X Cellular Wash Buffer, 15 mL, #6164
  • Fixative, 6 mL, #636
  • Kit Manual
  • Kit 950: 100 Tests
  • FAM-Leu-CMK, 4 vials, #6148
  • Hoechst Stain, 1 mL, #639
  • Propidium Iodide, 1 mL, #638
  • 10X Cellular Wash Buffer, 60 mL, #6165
  • Fixative, 6 mL, #636
  • Kit Manual
  • Product Specific References

    PMID Publication
    38127952Sabbione, F., et al. 2023. Regulation of human neutrophil IL-1β secretion induced by Escherichia coli O157:H7 responsible for hemolytic uremic syndrome. PLoS pathogens, e1011877.
    35861835Yang, S.F., et al. 2022. Chronic Kidney Disease Is Associated With Increased Cardiac Corin Expression But Decreased Proatrial Natriuretic Peptide Conversion Activity. Journal of the American Heart Association, e025208.

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