FL490 Antibody Labeling Kit

Name: FL490 Antibody Labeling Kit
Brand: Antibodies Incorporated
SKU: 81-0002
Price: 250.0 USD
Availability: InStock

This easy-to-use FL490 antibody labeling kit conjugates 50-100 μg of purified antibody (0.5-1.0 mg/mL) to FL490 in 10 minutes.

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Product Manual

Safety Data Sheet

SKU: 81-0002

Size: 1 kit

Variant

Price:

$250.00

Quantity:

Ships: 1-2 business days

Product Details

Scientific Background

To perform multiplex analysis for immunofluorescence (IF) or immunohistochemistry, antibody conjugates are often used but are not always commercially available. As an alternative option, and to support their research, scientists often create their own conjugates through selecting carrier free antibodies and conjugation kits which allows for flexibility to tailor design a set of conjugates (correct combination of antibodies with fluors, enzymes or biotin). The easy to use FL490 antibody labeling kits conjugates 50-100 μg of purified antibody (0.5-1.0 mg/mL) to FL490 in 10 minutes. Please note - for high labeling efficiency, the starting antibody concentration should be 0.5-1.1 mg/ml in buffer free of extraneous proteins such as BSA, serum or gelatin. Antibody formulations containing azide, trehalose or other sugars are acceptable. No tris buffer. Benefits of the kit include: Start to finish time is ~10 minutes and requires 15-30 seconds hands-on time. Antibody yield 95% to 10%. The kit is extremely easy to use - no prior conjugation experience is needed. Select from a diverse range of antibody labels. All components are included in each kit.

Usage Statement

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Country of Origin

United States

Expiration

12 months from date of receipt

Storage

Tube A, C, and D: 4°C
Tube B: -20°C

Shipping

Ships overnight (domestic), International Priority Shipping

Antibody Preparation 1) Add 1/10 volume of 10X modifier directly to your antibody tube (i.e. for 100 µl of antibody, add 10 µl of modifier). Labeling with FL490 2) Add antibody solution to the FL490 tube, mix thoroughly with pipette or vortex. Incubate in the dark at room temperature for 10 minutes. Final buffer step 3) After the 10-minute incubation is complete, add 90 µL final buffer diluent to FL490-labeled antibody, vortex, and it is now ready to use in your assay. Final antibody concentration is ~0.25-0.5 mg/mL, depending on starting concentration.

1. Modifier Tube. 2. Labeling reagent with lyophilized FL490 for modification of 50-100 µg protein at 0.5-1.0 mg/mL. 3. Desalting column (red cap) to buffer exchange FL490-modified antibody product into PBS + 15 mM sodium azide. 4. Final collection tube

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FL490 Antibody Labeling Kit

SKU: 81-0002

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