Products: Primary Antibodies

Anti-FGF14/FHF4 Antibody (N56/21)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-096	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-096	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

IHC IP

Target Description:

Fibroblast growth factor 14, FHF4 or FGF14 is encoded by FGF14. FHF4 is a member of the fibroblast growth factor homologous factors family (a subfamily of fibroblast growth factors.). FHFs have a role in regulation of voltage fated sodium channels (Nav channels) and are thought to be involved in nervous system development. FGF14 in found in both the hippocampus and the cerebellum localizes to the axon initial segment. Diseases associated with FGF14 include Spinocerebellar Ataxia and Autosomal Dominant Cerebellar Ataxia.

Immunogen:

Fusion protein amino acids 1-252 (full-length) of mouse FGF14b (also known as fibroblast growth factor homologous factor 4b or FHF4b, accession number O89096); Human: 98% identity (247/252 amino acids identical); Mouse: 100% identity (252/252 amino acids identical)

Cross Reactivity:

Cross-reacts with FGF14a and FGF14b Does not cross-react with FGF11, FGF12 or FGF13

Protein Name(s):

FGF14/FHF4

Gene Name(s):

Fgf14

Antibody Registry ID:

Purified: AB_2104060
TC Supernatant: AB_10671703

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

28 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.