Products: Primary Antibodies

Anti-FGF14/FHF4 (N56/21)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-096	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-096	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

IHC IP

Immunogen:

Fusion protein amino acids 1-252 (full-length) of mouse FGF14b (also known as fibroblast growth factor homologous factor 4b or FHF4b, accession number O89096)
Human: 98% identity (247/252 amino acids identical)
Mouse: 100% identity (252/252 amino acids identical)

Cross Reactivity:

Cross-reacts with FGF14a and FGF14b Does not cross-react with FGF11, FGF12 or FGF13

Protein Name(s):

Fibroblast growth factor (FGF)

Gene Name(s):

Fgf14

Antibody Registry ID:

Purified: AB_2104060
TC Supernatant: AB_10671703

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

28 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Citations and References

Bosch MK, Nerbonne JM,, Townsend RR,, Miyazaki H, Nukina N, Ornitz DM, Marionneau C.. (2016), 'Proteomic analysis of native cerebellar iFGF14 complexes. .' Channels (Austin).. 10.1080/19336950.2016.1153203.
Bosch MK, Carrasquillo Y, Ransdell JL, Kanakamedala A, Ornitz DM, Nerbonne JM.. (2015), 'Intracellular FGF14 (iFGF14) Is Required for Spontaneous and Evoked Firing in Cerebellar Purkinje Neurons and for Motor Coordination and Balance. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2663-14.2015.
Baalman K, Marin MA, Ho TS, Godoy M, Cherian L, Robertson C, Rasband MN.. (2015), 'Axon initial segment-associated microglia. .' Journal of Neuroscience. 10.1523/JNEUROSCI.3751-14.2015.
Yan H, Pablo JL, Wang C, Pitt GS.. (2014), 'FGF14 modulates resurgent sodium current in mouse cerebellar Purkinje neurons. .' Elife. 10.7554/eLife.04193.
Bosch MK, Nerbonne JM, Ornitz DM.. (2014), 'Dual transgene expression in murine cerebellar Purkinje neurons by viral transduction in vivo. .' PLoS One. 10.1371/journal.pone.0104062.
Puthussery T, Venkataramani S, Gayet-Primo J, Smith RG, Taylor WR.. (2013), 'NaV1.1 channels in axon initial segments of bipolar cells augment input to magnocellular visual pathways in the primate retina. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1249-13.2013.
Xiao M, Bosch MK, Nerbonne JM, Ornitz DM.. (2013), 'FGF14 localization and organization of the axon initial segment. .' Molecular and Cellular Neuroscience. 10.1016/j.mcn.2013.07.008.
Kaphzan H, Buffington SA, Jung JI, Rasband MN, Klann E.. (2013), 'Alterations in intrinsic membrane properties and the axon initial segment in a mouse model of Angelman syndrome. .' Journal of Neuroscience. 10.1523/JNEUROSCI.4162-11.2011.
Grubb MS, Burrone J.. (2010), 'Activity-dependent relocation of the axon initial segment fine-tunes neuronal excitability. .' Nature. 10.1038/nature09160.
Diwakar S, Magistretti J, Goldfarb M, Naldi G, D'Angelo E.. (2009), 'Axonal Na+ channels ensure fast spike activation and back-propagation in cerebellar granule cells. .' Journal of Neurophysiology. 10.1152/jn.90382.2008.
Shakkottai VG, Xiao M, Xu L, Wong M, Nerbonne JM, Ornitz DM, Yamada KA.. (2009), 'FGF14 regulates the intrinsic excitability of cerebellar Purkinje neurons. .' Neurobiology of Disease. 10.1016/j.nbd.2008.09.019.
Laezza F, Gerber BR, Lou JY, Kozel MA, Hartman H, Craig AM, Ornitz DM, Nerbonne JM.. (2007), 'The FGF14(F145S) mutation disrupts the interaction of FGF14 with voltage-gated Na+ channels and impairs neuronal excitability. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2282-07.2007.