Products: Primary Antibodies

Anti-FGF13/FHF2 (N91/27)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-134	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-134	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IP WB

Immunogen:

Fusion protein amino acids 53-245 of human FGF13a (also known as fibroblast growth factor homologous factor 2a or FHF2a, accession number Q92913)
Mouse: 99% identity (192/193 amino acids identical)
Rat: 97% identity (183/188 amino acids identical)
97% identity with FGF13b (184/188 amino acids identical)
>60% identity with FGF11, FGF12 and FGF14

Cross Reactivity:

Cross-reacts with FGF13a and FGF13b Does not cross-react with FGF11, FGF12 or FGF14 expressed in heterologous cells

Protein Name(s):

Fibroblast growth factor 13 (FGF-13) (Fibroblast growth factor homologous factor 2) (FHF-2)

Gene Name(s):

FGF13 FHF2

Antibody Registry ID:

Purified: AB_2104047
TC Supernatant: AB_10675138

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

28 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.