Products: Primary Antibodies

Anti-FGF12/FHF1 (N94/17)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-135	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-135	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC

Immunogen:

Fusion protein amino acids 1-243 (full-length) of human FGF12a (also known as fibroblast growth factor homologous factor 12a or FHF1a, accession number NP_066360) Rat: 100% identity (243/243 amino acids identical)
Mouse: 100% identity (243/243 amino acids identical)
60% identity with FGF11/FHF3, FGF13/FHF2 and FGF14/FHF4High identity with FGF12b/FHF1b

Cross Reactivity:

Cross-reacts with FGF12b/FHF1b Does not cross-react with FGF11/FHF3, FGF13/FHF2 or FGF14/FHF4

Protein Name(s):

Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor homologous factor 1) (FHF-1) (Myocyte-activating factor)

Gene Name(s):

FGF12 FGF12B FHF1

Antibody Registry ID:

Purified: AB_2278408
TC Supernatant: AB_10675137

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Wildburger NC, Ali SR, Hsu WC, Shavkunov AS, Nenov MN, Lichti CF, LeDuc RD, Mostovenko E, Panova-Elektronova NI, Emmett MR, Nilsson CL, Laezza F.. (2015), 'Quantitative proteomics reveals protein-protein interactions with fibroblast growth factor 12 as a component of the voltage-gated sodium channel 1.2 (nav1.2) macromolecular complex in Mammalian brain. .' Molecular & Cellular Proteomics. 10.1074/mcp.M114.040055.