Products: Primary Antibodies

Anti-FGF11/FHF3 (N92/14)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-133	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-133	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IP WB

Immunogen:

Fusion protein amino acids 55-216 of human FGF11a (also known as fibroblast growth factor homologous factor 3a or FHF3a number Q92914)
Mouse: 96% identity (157/162 amino acids identical)
Rat: 96% identity (156/162 amino acids identical)
>60% identity with FGF12, FGF13 and FGF14

Cross Reactivity:

Does not cross-react with FGF12, FGF13 or FGF14 in heterologous cells

Protein Name(s):

Fibroblast growth factor 11 (FGF-11) (Fibroblast growth factor homologous factor 3) (FHF-3)

Gene Name(s):

FGF11 FHF3

Antibody Registry ID:

Purified: AB_2103926
TC Supernatant: AB_10671702

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

25 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.