Products: Primary Antibodies

Anti-Dicer (N167/7)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-196	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-196	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer (also known as Dicer1, DCR, DCR-1, Double-strand-specific ribonuclease, Helicase with RNase motif, HERNA, Helicase MOI and KIAA0928, accession number Q8R418)
Human: 99% identity (257/262 amino acids identical)
Rat: 99% identity (257/262 amino acids identical)

Cross Reactivity:

None Reported

Protein Name(s):

Endoribonuclease Dicer (EC 3.1.26.3) (Double-strand-specific ribonuclease mDCR-1)

Gene Name(s):

Dicer1 Dicer Mdcr

Antibody Registry ID:

Purified: AB_2093085
TC Supernatant: AB_10673275

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Expected Banding Pattern:

215 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Hsu PK, Xu B, Mukai J, Karayiorgou M, Gogos JA.. (2015), 'The BDNF Val66Met variant affects gene expression through miR-146b. .' Neurobiology of Disease. 10.1016/j.nbd.2015.03.004.
Satterlee JS, Beckel-Mitchener A, McAllister K, Procaccini DC, Rutter JL, Tyson FL, Chadwick LH.. (2015), 'Community resources and technologies developed through the NIH Roadmap Epigenomics Program. .' Methods Mol Biol.. 10.1007/978-1-4939-1804-1_2.
Huang YW, Ruiz CR, Eyler EC, Lin K, Meffert MK.. (2012), 'Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis. .' Cell. 10.1016/j.cell.2012.01.036.