Products: Primary Antibodies

Anti-Dardarin/LRRK2, N3 (non-mouse-reactive) (N231B/34)

PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-266	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-266	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 20-60 ug/mL monoclonal antibody (mAb). Supernatant is suitable for most applications, including Western Blot, immunocytochemistry, immunohistochemistry, etc., and is a very economical choice compared to competitor offerings. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 841-960 of human LRRK2 (also known as Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin and PARK8, accession numberQ5S007)
Mouse: 81% identity (98/120 amino acids identical)
Rat: 80% identity (97/120 amino acids identical) 30% identity with LRRK1

Protein Name(s):

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

Gene Name(s):

LRRK2 PARK8

Antibody Registry ID:

Purified: AB_11000724
TC Supernatant: AB_11001669

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

>200 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.