Anti-LRRK2/Dardarin, N3 (Non-Mouse-Reactive) Antibody (N231B/34)

Name: Anti-LRRK2/Dardarin, N3 (Non-Mouse-Reactive) Antibody
Brand: NeuroMab
SKU: 75-266
Price: 319.0 USD
Availability: InStock

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Our Anti-Dardarin/LRRK2, N3 (non-mouse-reactive) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N231B/34. It is KO validated, detects human, mouse, and rat Dardarin/LRRK2, N3 (non-mouse-reactive), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against lysates of wildtype (WT) and LRRK2 knockout (KO) rat brains probed with N231B/34 (top) and anti- actin antibody (bottom). Reproduced with permission from the Proceedings of the National Academy of Sciences of the United States of America (2014 Daher et al, PMID 24927544).

Adult rat brain membrane immunoblot.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-266

Volume: 100 µL

Variant

Price:

$319

Quantity:

Product Details

Target

Dardarin/LRRK2, N3 (non-mouse-reactive)

Target Description

LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N231B/34

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

LRRK2 PARK8

Molecular Weight

>200 kDa

Antigen

Fusion protein amino acids 841-960 of human LRRK2 (accession number Q5S007) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_11000724

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

UniProt Details

UniProt (Human): Q5S007
UniProt (Immunogen Species): Q5S007

Choi I1,2,3, Kim B2, Byun JW1,2, Baik SH4, Huh YH5, Kim JH1,2, Mook-Jung I4, Song WK5, Shin JH6, Seo H7, Suh YH1,2,3, Jou I1,2,3, Park SM1,2,3, Kang HC8, Joe EH1,2,3,9,10. (2015), 'LRRK2 G2019S mutation attenuates microglial motility by inhibiting focal adhesion kinase..' Nat Commun. . 10.1038/ncomms9255.
Davies P1, Hinkle KM, Sukar NN, Sepulveda B, Mesias R, Serrano G, Alessi DR, Beach TG, Benson DL, White CL, Cowell RM, Das SS, West AB, Melrose HL. (2013), 'Comprehensive characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies..' Biochem J. 10.1042/BJ20121742.
Belluzzi E1,2, Gonnelli A3, Cirnaru MD4, Marte A5, Plotegher N6,7, Russo I8, Civiero L9, Cogo S10, Carrion MP11, Franchin C12,13, Arrigoni G14,15, Beltramini M16, Bubacco L17, Onofri F18, Piccoli G19,20, Greggio E21. (2016), 'LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate..' Mol Neurodegener. 10.1186/s13024-015-0066-z.
Daher JP1, Volpicelli-Daley LA1, Blackburn JP1, Moehle MS1, West AB2. (2014), 'Abrogation of α-synuclein-mediated dopaminergic neurodegeneration in LRRK2-deficient rats..' Proc Natl Acad Sci U S A. 10.1073/pnas.1403215111.
Dorval V1, Mandemakers W2, Jolivette F1, Coudert L3, Mazroui R3, De Strooper B2, Hébert SS1. (2014), 'Gene and MicroRNA transcriptome analysis of Parkinson's related LRRK2 mouse models..' PLoS One. 10.1371/journal.pone.0085510.

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Anti-LRRK2/Dardarin, N3 (Non-Mouse-Reactive) Antibody (N231B/34)

SKU: 75-266

Product Details

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