Anti-Clcn3 Antibody (N258/5)
Our Anti-Clcn3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N258/5. It is KO validated, detects human, mouse, and rat Clcn3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Human, Mouse, Rat
ICC, IHC, WB
Mouse
KO Validated
SKU: 75-259
Product Details
Clcn3
Chloride Voltage-Gated Channel 3, CLCN3 or H(+)/Cl(-) exchange transporter 3 is encoded by the gene CLCN3 and is a member of the voltage-gated chloride channel (ClC) family. CLCN3 is a proton-coupled chloride transporter. It plays a role in acidification of endosomes and synaptic vesicle lumen. CLCN3 implicated in vesicle trafficking, neuronal cell function and short term memory. CLCN3 is expressed in several tissues including brain, aortic and coronary vascular smooth muscle cells, bronchus and colon. Diseases associated with this gene include Dent Disease 1 and Cystic Fibrosis
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N258/5
IgG1
ICC, IHC, WB
Mouse
CLCN3
90 kDa
Synthetic peptide amino acids 98-115 (CKDRERHRRINSKKKESA, cytoplasmic N-terminus) of rat Clcn3 (accession number P51792)
Human, Mouse, Rat
AB_10671699
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Does not cross-react with Clcn4 or Clcn5 (based on KO validation results)
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
H(+)/Cl(-) exchange transporter 3 (Chloride channel protein 3) (ClC-3) (Chloride transporter ClC-3)
UniProt (Human): P51790
UniProt (Immunogen Species): P51790
UniProt (Immunogen Species): P51790
- Gaurav R1, Bewtra AK1, Agrawal DK1. (2014), 'Novel CLC3 transcript variants in blood eosinophils and increased CLC3 expression in nasal lavage and blood eosinophils of asthmatics..' Immun Inflamm Dis. 10.1002/iid3.36.
- Cuddapah VA1, Turner KL, Sontheimer H. (2013), 'Calcium entry via TRPC1 channels activates chloride currents in human glioma cells..' Cell Calcium. 10.1016/j.ceca.2012.11.013.
- McMains E1, Krishnan V, Prasad S, Gleason E. (2011), 'Expression and localization of CLC chloride transport proteins in the avian retina..' PLoS One. 10.1371/journal.pone.0017647.