Human Mouse Rat
ICC IHC WB
Chloride Voltage-Gated Channel 3, CLCN3 or H(+)/Cl(-) exchange transporter 3 is encoded by the gene CLCN3 and is a member of the voltage-gated chloride channel (ClC) family. CLCN3 is a proton-coupled chloride transporter. It plays a role in acidification of endosomes and synaptic vesicle lumen. CLCN3 implicated in vesicle trafficking, neuronal cell function and short term memory. CLCN3 is expressed in several tissues including brain, aortic and coronary vascular smooth muscle cells, bronchus and colon. Diseases associated with this gene include Dent Disease 1 and Cystic Fibrosis
Synthetic peptide amino acids 98-115 (CKDRERHRRINSKKKESA, cytoplasmic N-terminus) of rat Clcn3 (also known as H(+)/Cl(-) exchange transporter 3, Chloride transporter ClC-3, Chloride channel protein 3 and Clc3, accession number P51792)
Mouse: 100% identity (18/18 amino acids identical)
Human: 100% identity (18/18 amino acids identical) <70% identity with Clcn4 and Clcn5
Does not cross-react with Clcn4 or Clcn5 (based on KO validation results)
H(+)/Cl(-) exchange transporter 3 (Chloride channel protein 3) (ClC-3) (Chloride transporter ClC-3)
TC Supernatant: AB_10673153
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Citations and References
- Gaurav R1, Bewtra AK1, Agrawal DK1.. (2014), 'Novel CLC3 transcript variants in blood eosinophils and increased CLC3 expression in nasal lavage and blood eosinophils of asthmatics..' Immun Inflamm Dis.. 10.1002/iid3.36.
- Cuddapah VA1, Turner KL, Sontheimer H.. (2013), 'Calcium entry via TRPC1 channels activates chloride currents in human glioma cells..' Cell Calcium.. 10.1016/j.ceca.2012.11.013.
- McMains E1, Krishnan V, Prasad S, Gleason E.. (2011), 'Expression and localization of CLC chloride transport proteins in the avian retina..' PLoS One.. 10.1371/journal.pone.0017647.