Products: Primary Antibodies

Anti-CDY1/2 Antibody (N143/38)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-174	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-174	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human

Applications:

ICC WB

Target Description:

This antibody recognizes the proteins Testis-specific chromodomain protein Y 1 and 2 and are encoded by the genes CDY1 and CDY2A respectively. These genes each have a paralog (CDY1B and CDY2B) which are also present on the Y chromosome. Proteins in the CDY-related family contain a chromodomain, involved in chromatin binding, and a histone acetyltransferase catalytic domain. These proteins are expressed in the nucleaus in testis. Diseases associated with these genes include Y-Linked Spermatogenic Failure, and Y Chromosome Infertility.

Immunogen:

Fusion protein amino acids 6-66 (Chromo domain) of human CDY1 (also known as Testis-specific chromodomain protein Y 1, accession number Q9Y6F8)
100% identity with human CDY273% identity with human CDYL1 (44/60 amino acids identical)
51% identity with human CDYL2 (31/60 amino acids identical)

Cross Reactivity:

No cross-reactivity against CDYL1 or CDYL2

Protein Name(s):

CDY1/2

Gene Name(s):

CDY1 CDY1A; CDY1B

Antibody Registry ID:

Purified: AB_10673274
TC Supernatant: AB_10673152

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

60 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.