Products: Primary Antibodies

Anti-Cav1.3 Ca2+ channel (L48A/9)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-113	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-113	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Synthetic peptide amino acids 859-875 (N-terminus) of rat Cav1.3 (also known as voltage-dependent L-type calcium channel subunit alpha-1D, RAT brain class D and RBD, accession number P27732)
Mouse: 94% identity (16/17 amino acids identical)
Human: 88% identity (15/17 amino acids identical)

Cross Reactivity:

No cross-reactivity against Cav1.2

Protein Name(s):

Voltage-dependent L-type calcium channel subunit alpha-1D (Calcium channel, L type, alpha-1 polypeptide, isoform 2) (Rat brain class D) (RBD) (Voltage-gated calcium channel subunit alpha Cav1.3)

Gene Name(s):

Cacna1d Cach3 Cacn4 Cacnl1a2 Cchl1a2

Antibody Registry ID:

Purified: AB_2069388
TC Supernatant: AB_10672112

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

250 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Perissinotti PP, Ethington EA, Almazan E, Martínez-Hernández E, Kalil J, Koob MD, Piedras-Rentería ES.. (2015), 'Calcium current homeostasis and synaptic deficits in hippocampal neurons from Kelch-like 1 knockout mice. .' Frontiers in Neuroscience. 10.3389/fncel.2014.00444.
Lu L, Sirish P, Zhang Z, Woltz RL, Li N, Timofeyev V, Knowlton AA, Zhang XD, Yamoah EN, Chiamvimonvat N.. (), 'Regulation of gene transcription by voltage-gated L-type calcium channel, Cav1.3. .' The Journal of Biological Chemistry. 10.1074/jbc.M114.586883.
Perissinotti PP, Ethington EG, Cribbs L, Koob MD, Martin J, Piedras-Rentería ES.. (2014), 'Down-regulation of endogenous KLHL1 decreases voltage-gated calcium current density. .' Cell Calcium. 10.1016/j.ceca.2014.03.002.
Dryanovski DI, Guzman JN, Xie Z, Galteri DJ, Volpicelli-Daley LA, Lee VM, Miller RJ, Schumacker PT, Surmeier DJ.. (2013), 'Calcium entry and α-synuclein inclusions elevate dendritic mitochondrial oxidant stress in dopaminergic neurons. .' Journal of Neuroscience. 10.1523/JNEUROSCI.5311-12.2013.
Zou J, Lee A, Yang J.. (), 'The expression of whirlin and Cav1.3α₁ is mutually independent in photoreceptors. .' Vision Research. 10.1016/j.visres.2012.07.020.
Yamasaki S, Hasegawa A, Hojyo S, Ohashi W, Fukada T, Nishida K, Hirano T.. (2012), 'A novel role of the L-type calcium channel α1D subunit as a gatekeeper for intracellular zinc signaling: zinc wave. .' PLoS One. 10.1371/journal.pone.0039654.
Comerota AJ. (2011), 'Invited commentary. .' Journal of Vascular Surgery. 10.1016/j.jvs.2011.06.017.
Goonasekera SA, Hammer K, Auger-Messier M, Bodi I, Chen X, Zhang H, Reiken S, Elrod JW, Correll RN, York AJ, Sargent MA, Hofmann F, Moosmang S, Marks AR, Houser SR, Bers DM, Molkentin JD.. (2012), 'Decreased cardiac L-type Ca²⁺ channel activity induces hypertrophy and heart failure in mice. .' Journal of Clinical Investigation. 10.1172/JCI58227.
Hettiarachchi NT, Parker A, Dallas ML, Pennington K, Hung CC, Pearson HA, Boyle JP, Robinson P, Peers C.. (2009), 'alpha-Synuclein modulation of Ca2+ signaling in human neuroblastoma (SH-SY5Y) cells. .' Journal of Neurochemistry. 10.1111/j.1471-4159.2009.06411.x.
Abiria SA, Colbran RJ.. (2010), 'CaMKII associates with CaV1.2 L-type calcium channels via selected beta subunits to enhance regulatory phosphorylation. .' Journal of Neurochemistry. 10.1111/j.1471-4159.2009.06436.x.