Products: Primary Antibodies

Anti-CASK (K56A/50)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-000	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-000	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with K56A/50 (left) or N52A/42 (right) TC supe.$

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat Xenopus Zebrafish

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 318-415 of rat CASK/Lin2 (accession number Q62915)
Human: 100% identity
Mouse=100% identity

Cross Reactivity:

None Reported

Protein Name(s):

Peripheral plasma membrane protein CASK (hCASK) (EC 2.7.11.1) (Calcium/calmodulin-dependent serine protein kinase) (Protein lin-2 homolog)

Gene Name(s):

CASK LIN2

Antibody Registry ID:

Purified: AB_2068730
TC Supernatant: AB_10671954

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Gao R, Piguel NH, Melendez-Zaidi AE, Martin-de-Saavedra MD, Yoon S, Forrest MP, Myczek K, Zhang G, Russell TA, Csernansky JG, Surmeier DJ, Penzes P. (2018), 'CNTNAP2 stabilizes interneuron dendritic arbors through CASK..' Mol Psychiatry Journal. 10.1038/s41380-018-0027-3.
Kamijo A, Saitoh Y, Ohno N, Ohno S, Terada N. (2016), 'Immunohistochemical study of the membrane skeletal protein, membrane protein palmitoylated 6 (MPP6), in the mouse small intestine..' Histochemistry and Cell Biology. 10.1007/s00418-015-1374-7.
Andrews RE, Galileo DS, Martin-DeLeon PA. (2015), 'Plasma membrane Ca2+-ATPase 4: interaction with constitutive nitric oxide synthases in human sperm and prostasomes which carry Ca2+/CaM-dependent serine kinase..' Molecular Human Reproduction. 10.1093/molehr/gav049.
Tindi JO, Chávez AE, Cvejic S, Calvo-Ochoa E, Castillo PE, Jordan BA. (2015), 'ANKS1B Gene Product AIDA-1 Controls Hippocampal Synaptic Transmission by Regulating GluN2B Subunit Localization..' Journal of Neuroscience. 10.1523/JNEUROSCI.4029-14.2015.
Fernandes HB, Riordan S, Nomura T, Remmers CL, Kraniotis S, Marshall JJ, Kukreja L, Vassar R, Contractor A. (2015), 'Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus..' Journal of Neuroscience. 10.1523/JNEUROSCI.0314-14.2015.
de Andrade GB, Kunzelman L, Merrill MM, Fuerst PG. (2014), 'Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina..' Molecular Vision. .
Anjum R, Ayoubian H, Schmitz F. (2014), 'Differential synaptic distribution of the scaffold proteins Cask and Caskin1 in the bovine retina..' Molecular and Cellular Neuroscience. 10.1016/j.mcn.2014.08.004.
Lugo JN, Smith GD, Arbuckle EP, White J, Holley AJ, Floruta CM, Ahmed N, Gomez MC, Okonkwo O. (2014), 'Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins..' Frontiers in Molecular Neuroscience. 10.3389/fnmol.2014.00027.
Li Q, Zheng S, Han A, Lin CH, Stoilov P, Fu XD, Black DL. (2014), 'The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation..' Elife. 10.7554/eLife.01201.
Green JA, Yang J, Grati M, Kachar B, Bhat MA. (2013), 'Whirlin, a cytoskeletal scaffolding protein, stabilizes the paranodal region and axonal cytoskeleton in myelinated axons..' BMC Neuroscience. 10.1186/1471-2202-14-96.
Wentzel C, Sommer JE, Nair R, Stiefvater A, Sibarita JB, Scheiffele P. (2013), 'mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking..' Neuron. 10.1016/j.neuron.2013.05.010.
Patel R, Al-Dossary AA, Stabley DL, Barone C, Galileo DS, Strehler EE, Martin-DeLeon PA. (2013), 'Plasma membrane Ca2+-ATPase 4 in murine epididymis: secretion of splice variants in the luminal fluid and a role in sperm maturation..' Biology of Reproduction. 10.1095/biolreprod.113.108712.
Boyken J, Grønborg M, Riedel D, Urlaub H, Jahn R, Chua JJ. (2013), 'Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses..' Neuron. 10.1016/j.neuron.2013.02.027.
Cheadle L, Biederer T. (2012), 'The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization..' The Journal of Biology. 10.1083/jcb.201205041.
Aravindan RG, Fomin VP, Naik UP, Modelski MJ, Naik MU, Galileo DS, Duncan RL, Martin-Deleon PA. (2012), 'CASK interacts with PMCA4b and JAM-A on the mouse sperm flagellum to regulate Ca2+ homeostasis and motility..' Journal of Cellular Physiology. 10.1002/jcp.24000.
Robbins EM, Krupp AJ, Perez de Arce K, Ghosh AK, Fogel AI, Boucard A, Südhof TC, Stein V, Biederer T. (2010), 'SynCAM 1 adhesion dynamically regulates synapse number and impacts plasticity and learning..' Neuron. 10.1016/j.neuron.2010.11.003.
Wahlin KJ, Hackler L Jr, Adler R, Zack DJ. (2010), 'Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina..' Journal of Comparative Neurology. 10.1002/cne.22499.
Stagi M, Fogel AI, Biederer T. (2010), 'SynCAM 1 participates in axo-dendritic contact assembly and shapes neuronal growth cones..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.0911798107.
Klemmer P, Smit AB, Li KW. (2009), 'Proteomics analysis of immuno-precipitated synaptic protein complexes..' Journal of Proteomics. 10.1016/j.jprot.2008.10.005.
Wahlin KJ, Moreira EF, Huang H, Yu N, Adler R. (2008), 'Molecular dynamics of photoreceptor synapse formation in the developing chick retina..' Journal of Comparative Neurology. 10.1002/cne.21582.
Fischer AJ, Foster S, Scott MA, Sherwood P. (2008), 'Transient expression of LIM-domain transcription factors is coincident with delayed maturation of photoreceptors in the chicken retina..' Journal of Comparative Neurology. 10.1002/cne.21578.