Non ionic detergents like Brij 35 are used in immunoassays for diluting antibodies to lower background, and non-specific ag-ab reaction. This detergent is also used in washing buffer in all immunoassays (ELISA, IHC, Blot assays and others) to reduce background. Brij 35 is polyoxyethylene laural ether, it lowers surface tension of water.
Non ionic detergents like Brij 35 are used in immunoassays for diluting antibodies to lower background, and non-specific ag-ab reaction. This detergent is also used in washing buffer from 0.5- 0.1% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Brij 35 is polyoxyethylene laural ether, it lowers surface tension of water.
ELISA, IHC
This detergent is supplied as 15% in water with 0.15 % Proclin 300 as preservative.
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
Room Temperature
Ships overnight (domestic), International Priority Shipping
For diluting antibodies use approximately 1:400 (1 ml into 399 ml) or more dilution.
As washing buffer use 1:150 (1 ml into 149 ml buffer) or 1:300 (1 ml into
299 ml).
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