Bovine A2 Beta casein (A2) ELISA Assay

Name: Bovine A2 Beta casein (A2) ELISA Assay
Brand: Biosensis
SKU: BEK-2244-2P
Price: 847.0 USD
Availability: InStock

The Biosensis Bovine A2 β-casein ELISA Kit is a sandwich ELISA that allows the quantification of Bovine A2 β-casein in 5 hours. This Bovine A2 β-casein ELISA kit employs a purified, native A2 β-casein protein as a standard. This ELISA assay does not cross-react with Bovine A1 β-casein proteins.

Parallelism in bovine milk samples demonstrates accurate quantification of A2 beta-casein.

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

Bovine A1 beta-casein does not cross-react in this ELISA assay.

Parallelism in bovine milk samples demonstrates accurate quantification of A2 beta-casein.

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Species Reactivity

Bovine

Applications

ELISA

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Data Sheet

SKU: BEK-2244-2P

Volume/Size: 2 Plate Kit

Variant

Price:

$847.00

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Product Description

β-casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are β-casein A1 and β-casein A2. The two types of β-casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 β-casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 β-casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine β-casein polyclonal capture antibody, a chicken anti-bovine A2 β-casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 β-casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 β-casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.

Target

A2 Beta casein (A2)

Immunogen

Purified bovine beta Casein and peptide specific peptides for A2

Immunogen Species

Bovine

Range

3.1 - 200 ng/mL

Sensitivity

This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))

Specificity

Bovine A2 beta-casein. No cross-reactivity is observed with bovine A1 beta-Casein.

Species Reactivity

Bovine

Applications

ELISA

Method of Analysis

Colorimetric

Sample Type

Bovine Milk

Synonyms

CSN2; Bovine A2 Beta Casein; A2

UniProt ID

P02666

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt unopened.

Storage

Store at 2-8°C

Shipping

Shipped on ice packs

Bovine A2 Beta casein (A2) ELISA Assay Protocol
Technical Note 1 - ELISA Validation
Technical Note 2 - ELISA Data Analysis
Calculation of Antigen Amount in Tissues and Lysates

Reagent	1 Plate Kit	2 Plate Kit
Bovine β-casein Antibody Coated 96 well microplate	1 plate	2 plates
Assay Diluent B, 1X	25 mL x 2	25 mL x 4
Bovine A2 β-casein Standard	200 ng x 2	200 ng x 4
Bovine A2 β-casein Specific Detection Antibody (Lyophilized)	1 Vial	2 Vials
Anti-Chicken IgY-HRP Conjugate, 100X	110 μL x 1	110 μL x 2
Wash Buffer, 10X	33 mL x 1	33 mL x 2
TMB Substrate, 1X	11 mL x 1	11 mL x 2
Stop Solution, 1X	11 mL x 1	11 mL x 2
Plate sealer	Supplied

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Bovine A2 Beta casein (A2) ELISA Assay

SKU: BEK-2244-2P

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