Bovine A1 Beta casein (A1) ELISA Assay

Name: Bovine A1 Beta casein (A1) ELISA Assay
Brand: Biosensis
SKU: BEK-2243-2P
Price: 787.0 USD
Availability: InStock

The Biosensis Bovine A1 β-Casein ELISA Kit is a sandwich ELISA that allows the quantification of Bovine A1 β-Casein in 5 hours. This Bovine A1 β-casein ELISA kit employs a purified, native A1 β-casein protein as a standard. It does not cross-react with Bovine A2 β-casein proteins.

Parallelism in bovine milk samples demonstrates accurate quantification of A1 beta-casein.

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

Bovine A2 beta-casein does not cross-react in this ELISA assay.

Parallelism in bovine milk samples demonstrates accurate quantification of A1 beta-casein.

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Species Reactivity

Bovine

Applications

ELISA

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Data Sheet

SKU: BEK-2243-2P

Volume/Size: 2 Plate Kit

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Product Description

β-casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are β-casein A1 and β-casein A2. The two types of β-casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 β-casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 β-casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine β-casein polyclonal capture antibody, a chicken anti-bovine A1 β-casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 β-casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 β-casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.

Target

A1 Beta casein (A1)

Immunogen

Purified bovine beta Casein and peptide specific peptides for A1

Immunogen Species

Bovine

Range

3.1 - 200 ng/mL

Sensitivity

This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))

Specificity

Bovine A1 beta-casein. No cross-reactivity is observed with bovine A2 beta-Casein.

Species Reactivity

Bovine

Applications

ELISA

Method of Analysis

Colorimetric

Sample Type

Bovine Milk

Synonyms

CSN2; Bovine A1 Beta Casein; A1

UniProt ID

P02666

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt unopened.

Storage

Store at 2-8°C

Shipping

Shipped on ice packs

Bovine A1 Beta casein (A1) ELISA Assay Protocol
Technical Note 1 - ELISA Validation
Technical Note 2 - ELISA Data Analysis
Calculation of Antigen Amount in Tissues and Lysates

Reagent	1 Plate Kit	2 Plate Kit
Bovine β-casein Antibody Coated 96 well microplate	1 plate	2 plates
Assay Diluent B, 1X	25 mL x 2	25 mL x 4
Bovine A1 β-casein Standard	200 ng x 2	200 ng x 4
Bovine A1 β-casein Specific Detection Antibody (Lyophilized)	1 Vial	2 Vials
Anti-Chicken IgY-HRP Conjugate, 100X	110 μL x 1	110 μL x 2
Wash Buffer, 10X	33 mL x 1	33 mL x 2
TMB Substrate, 1X	11 mL x 1	11 mL x 2
Stop Solution, 1X	11 mL x 1	11 mL x 2
Plate sealer	Supplied

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Bovine A1 Beta casein (A1) ELISA Assay

SKU: BEK-2243-2P

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