Products: Primary Antibodies

Anti-Botch (N116/14)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-181	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-181	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 1-222 (full-length) of rat Botch1 (also known as Cation transport regulator-like protein 1 or Chac1, accession number XM_342497.1)
Mouse: 96% identity (215/222 amino acids identical)
Human: 89% identity (198/222 amino acids identical)
>50% identity with Botch2/Chac2

Cross Reactivity:

No cross-reactivity against Botch2/Chac2

Protein Name(s):

Glutathione-specific gamma-glutamylcyclotransferase 1 (Gamma-GCG 1) (EC 4.3.2.7) (Blocks Notch protein) (Botch) (Cation transport regulator-like protein 1) (Neuroprotective protein 7)

Gene Name(s):

Chac1 Botch Npg7

Antibody Registry ID:

Purified: AB_2079324
TC Supernatant: AB_10671697

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

25 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Chi Z, Byrne ST, Dolinko A, Harraz MM, Kim MS, Umanah G, Zhong J, Chen R, Zhang J, Xu J, Chen L, Pandey A, Dawson TM, Dawson VL.. (2014), 'Botch is a γ-glutamyl cyclotransferase that deglycinates and antagonizes Notch. .' Cell Report. 10.1016/j.celrep.2014.03.048.
Chi Z, Zhang J, Tokunaga A, Harraz MM, Byrne ST, Dolinko A, Xu J, Blackshaw S, Gaiano N, Dawson TM, Dawson VL.. (2012), 'Botch promotes neurogenesis by antagonizing Notch. .' Developmental Cell. 10.1016/j.devcel.2012.02.011.