Human Mouse Rat
Fusion protein amino acids 1-222 (full-length) of rat Botch1 (also known as Cation transport regulator-like protein 1 or Chac1, accession number XM_342497.1)
Mouse: 96% identity (215/222 amino acids identical)
Human: 89% identity (198/222 amino acids identical)
>50% identity with Botch2/Chac2
No cross-reactivity against Botch2/Chac2
Glutathione-specific gamma-glutamylcyclotransferase 1 (Gamma-GCG 1) (EC 126.96.36.199) (Blocks Notch protein) (Botch) (Cation transport regulator-like protein 1) (Neuroprotective protein 7)
Chac1 Botch Npg7
TC Supernatant: AB_10671697
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.
Citations and References
- Chi Z, Byrne ST, Dolinko A, Harraz MM, Kim MS, Umanah G, Zhong J, Chen R, Zhang J, Xu J, Chen L, Pandey A, Dawson TM, Dawson VL.. (2014), 'Botch is a γ-glutamyl cyclotransferase that deglycinates and antagonizes Notch. .' Cell Report. 10.1016/j.celrep.2014.03.048. " "
- Chi Z, Zhang J, Tokunaga A, Harraz MM, Byrne ST, Dolinko A, Xu J, Blackshaw S, Gaiano N, Dawson TM, Dawson VL.. (2012), 'Botch promotes neurogenesis by antagonizing Notch. .' Developmental Cell. 10.1016/j.devcel.2012.02.011. "