Products: Primary Antibodies

Anti-Botch Antibody (N116/14)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-181
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-181
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC WB

Target Description:

Cation transport regulator-like protein 1, Glutathione-Specific Gamma-Glutamylcyclotransferase 1 or Botch1 is encoded by the gene CHAC1. Chac1 is a member of the gamma-glutamylcyclotransferase family. It is an enzyme that acts to catalyze the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Chac1 is involved in neuronal differentiation by deglycination of the Notch receptor, which prevents receptor maturation and inhibits Notch signaling. CHAC1 is expressed in tissues including brain (forebrain and anterior spinal cord), and heart. Diseases associated with CHAC1 include Ovarian Embryonal Carcinoma and Ovarian Cancer.

Immunogen:

Fusion protein amino acids 1-222 (full-length) of rat Botch1 (also known as Cation transport regulator-like protein 1 or Chac1, accession number B3STU3.1); Mouse: 96% identity (215/222 amino acids identical); Human: 89% identity (198/222 amino acids identical); Greater than 50% identity with Botch2/Chac2

Cross Reactivity:

No cross-reactivity against Botch2/Chac2

Protein Name(s):

Botch

Gene Name(s):

Chac1 Botch Npg7

Antibody Registry ID:

Purified: AB_2079324
TC Supernatant: AB_10671697


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

25 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.