Products: Primary Antibodies

Anti-BKbeta3a K+ channel (N40B/18)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-098	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-098	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 1-49 (unique N-terminus) of mouse BKBeta3a (accession number XP_989315)
Human: 86% identity (42/49 amino acids identical)
Rat: not in database

Cross Reactivity:

No cross-reactivity against BKBeta1, BKBeta2, BKBeta3b or BKBeta4

Protein Name(s):

Potassium large conductance calcium-activated channel, subfamily M, beta member 3

Gene Name(s):

Kcnmb3

Antibody Registry ID:

Purified: AB_10672110
TC Supernatant: AB_10673961

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

32 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Linley J, Loganathan A, Kopanati S, Sandle GI, Hunter M.. (2014), 'Evidence that two distinct crypt cell types secrete chloride and potassium in human colon. .' Gut. 10.1136/gutjnl-2013-304695.
Zhang XL, Mok LP, Lee KY, Charbonnet M, Gold MS.. (), 'Inflammation-induced changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the adult rat. .' Molecular Pain. 10.1186/1744-8069-8-37.