Biotin Antibody Labeling Kit
This easy-to-use biotin antibody labeling kit conjugates 50-100 μg of purified antibody (0.5-1.0 mg/mL) to biotin in 10 minutes. The kit also includes a ready-to-use spin filter to rapidly remove >80% of biotin from the conjugated antibody, with minimal loss (optional step). All components required to label an antibody are included in the kit.
Biotin Antibody Labeling Kit
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Ships: 1-2 business days
Antibody Preparation:
1) Add 1/10 volume of 10X modifier directly to your antibody tube (i.e. for 100 μl of antibody, add 10 μl of modifier).
Labeling with biotin
2) Add antibody solution to the biotin tube, mix thoroughly with pipette or vortex. Incubate in the dark at room temperature for 10 minutes.
Final buffer step
3) After the 10-minute incubation is complete, add 90 μL final buffer diluent to biotin/fluor-labeled antibody, vortex, and it is now ready to use. Final antibody concentration is ~0.25-0.5 mg/mL, depending on starting concentration.
Notes
• For biotin conjugation it is highly recommended for optimal performance to proceed to the PURIFICATION STEP below following the 10-minute incubation with final buffer.
Optional Purification Step
(a) PBS/azide desalting column preparation - Break off bottom of RED-capped column and loosen the RED cap, place column in a collection tube, then place inmicrocentrifuge and spin for 2 minutes at 1500 g. Discard flow through.
(b) Transfer probe labeled mixture to the RED-capped column, place column in a new collection tube and centrifuge for 2 minutes at 1500 g.
(c) Transfer desalted fluor labeled antibody to a new capped tube for 4ºC storage and you now have a biotin free, labeled sample ready to use.
1. Modifier Tube.
2. Labeling reagent with lyophilized Peg4-biotin for modification of 50-100 µg protein at 0.5-1.0 mg/mL.
3. Desalting column (red cap) to buffer exchange biotin-modified antibody product into PBS + 15 mM sodium azide.
4. Final collection tube.