Products: Primary Antibodies

Anti-Beta4-spectrin (N393/76)


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PRODUCT OPTIONS

Catalog # Option Concentration Price*
Catalog #
75-377
Option
Purified
Concentration
1 mg/mL
Price
$205.00
Catalog #
73-377
Option
TC Supernatant
Concentration
Lot dependent
Price
$180.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (also known as BetaIV-spectrin sigma1, Nonerythrocytic spectrin beta chain 4, Non-erythroid spectrin beta chain 3, SPTBN4, SPTBN3, Spnb4, KIAA1642, EMBL BAB83243.1 and Ensembl ENSRNOP00000028393, accession number Q9H254)
Mouse: 98% identity (209/212 amino acids identical)
Rat: 98% identity (209/212 amino acids identical)
60% identity with Beta2-spectrin

Cross Reactivity:

Does not cross-react with other Beta-spectrins

Protein Name(s):

Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)

Gene Name(s):

SPTBN4 KIAA1642 SPTBN3

Antibody Registry ID:

Purified: AB_2315818
TC Supernatant: AB_2315817


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

>200 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.