Human Mouse Rat
ICC IHC WB
Spectrin beta chain, non-erythrocytic 4, or ß4 spectrin, is a member of the spectrin family of cytoskeletal proteins. It is found in the cytoskeleton and cell cortex of cells in the brain and pancreatic islets. It is also expressed in skeletal muscule. ß4 spectrin functions as a scaffold protein that links the actin cytoskeleton to the plasma membrane. It is involved in cell shape and organization. Mutations in the ß4 spectrin gene are associated with congenital myopathy, and neurodevelopmental disorder with hypotonia, neuropathy and deafness (NEDHND).
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (also known as BetaIV-spectrin sigma1, Nonerythrocytic spectrin beta chain 4, Non-erythroid spectrin beta chain 3, SPTBN4, SPTBN3, Spnb4, KIAA1642, EMBL BAB83243.1 and Ensembl ENSRNOP00000028393, accession number Q9H254)
Mouse: 98% identity (209/212 amino acids identical)
Rat: 98% identity (209/212 amino acids identical)
60% identity with Beta2-spectrin
Does not cross-react with other Beta-spectrins
Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)
SPTBN4 KIAA1642 SPTBN3
TC Supernatant: AB_2315817
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.