Human Mouse Rat
IHC IP WB
Synthetic peptide amino acids 164-197 (ATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPP) of mouse ataxin-1 (also known as spinocerebellar ataxia type 1 protein homolog, accession number P54254)
Rat: 100% identity (34/34 amino acids identical)
Human: 88% identity (30/34 amino acids identical)
Ataxin-1 (Spinocerebellar ataxia type 1 protein homolog)
TC Supernatant: AB_10671696
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.
Citations and References
- Venkatraman A, Hu YS, Didonna A, Cvetanovic M, Krbanjevic A, Bilesimo P, Opal P.. (2014), 'The histone deacetylase HDAC3 is essential for Purkinje cell function, potentially complicating the use of HDAC inhibitors in SCA1. .' Human Molecular Genetics. 10.1093/hmg/ddu081. " "
- Bergeron D, Lapointe C, Bissonnette C, Tremblay G, Motard J, Roucou X.. (2013), 'An out-of-frame overlapping reading frame in the ataxin-1 coding sequence encodes a novel ataxin-1 interacting protein. .' The Journal of Biological Chemistry. 10.1074/jbc.M113.472654. " "
- Park J#,,, Al-Ramahi I#,, Tan Q,,, Mollema N,, Diaz-Garcia JR,, Gallego-Flores T,, Lu HC,, Lagalwar S,, Duvick L,, Kang H,, Lee Y,,, Jafar-Nejad P,, Sayegh LS,, Richman R,,, Liu X,,, Gao Y,, Shaw CA, Arthur JSC, Orr HT,, Westbrook TF,,, Botas J,, Zoghbi HY,,.. (), 'RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1. .' Nature. 10.1038/nature12204. " "
- Zhang C, Browne A, Child D, Divito JR, Stevenson JA, Tanzi RE.. (2010), 'Loss of function of ATXN1 increases amyloid beta-protein levels by potentiating beta-secretase processing of beta-amyloid precursor protein. .' The Journal of Biological Chemistry. 10.1074/jbc.M109.079079. "