Products: Primary Antibodies

Anti-ASIC1 (N271/44)


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PRODUCT OPTIONS

Catalog # Option Concentration Price*
Catalog #
75-277
Option
Purified
Concentration
1 mg/mL
Price
$205.00
Catalog #
73-277
Option
TC Supernatant
Concentration
Lot dependent
Price
$180.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Fusion protein amino acids 460-526 (cytoplasmic C-terminus) of mouse ASIC1 (also known as Acid-sensing ion channel 1, Neuronal amiloride-sensitive cation channel 2, Brain sodium channel 2, BNaC2 and Accn2, accession number Q6NXK8)
Rat: 98% identity (66/67 amino acids identical)
Human: 97% identity (65/67 amino acids identical) 100% identity between ASIC1a and ASIC1b40% identity with ASIC4

Cross Reactivity:

Does not cross-react with ASIC2

Protein Name(s):

Acid-sensing ion channel 1 (ASIC1) (Acid-sensing ion channel) (Amiloride-sensitive cation channel 2, neuronal) (Brain sodium channel 2) (BNaC2)

Gene Name(s):

Asic1 Accn2 Asic Bnac2

Antibody Registry ID:

Purified: AB_11030370
TC Supernatant: AB_11000719


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

60 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.