Anti-ASIC1 Antibody (N271/44)

Name: Anti-ASIC1 Antibody
Brand: NeuroMab
SKU: 75-277
Price: 337.0 USD
Availability: InStock

Our mouse ASIC1 monoclonal antibody from NeuroMab is produced in-house from clone N271/44. It detects human, mouse, and rat ASIC1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ASIC1, ASIC2 or untagged Kv2.1 plasmid and probed with N271/44 (left) or N86/8 (right) TC supe.

Immunoblot versus whole brain lysates from adult WT, ASIC1 KO and ASIC2 KO mice probed with N271/44 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Julie Saugstad (Robert S. Dow Neurobiology Laboratories, Legacy Research Institute).

Representative digitally generated blot of ASIC1 (Cat. 75-277, 1:100) in dorsal root ganglion (DRG) tissue isolated from TRPV1+/+, TRPV1+/− and TRPV1−/− rats. For ASIC1 the bands shown at 64 and 89 kDa represent the non-glycosylated and glycosylated forms of ASIC1, respectively. There is no significant change in the expression of ASIC1 between the three groups. Image from publication CC-BY-4.0. PMID:37833914

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ASIC1, ASIC2 or untagged Kv2.1 plasmid and probed with N271/44 (left) or N86/8 (right) TC supe.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-277

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

ASIC1

Scientific Background

Acid sensing ion channel 1, Neuronal amiloride-sensitive cation channel 2 or ASIC1 is encoded by the gene ASIC1 and is a member of the Acid sensing ion channel (ASIC) family. ASIC1 acts as an acid sensing proton gated sodium channel and is thought to play a role in learning, pain transduction, memory development, and fear conditioning. ASIC1 is widely expressed in the central and peripheral nervous system. Diseases associated with ASIC1 include spatial learning and memory capability problems and Frozen Shoulder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N271/44

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Asic1 Accn2 Asic Bnac2

Molecular Weight

60 kDa

Immunogen

Fusion protein amino acids 460-526 (cytoplasmic C-terminus) of mouse ASIC1 (accession number Q6NXK8) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_11030370

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:1000
IP: 1.5 µL

Conjugate

Unconjugated

Specificity

Does not cross-react with ASIC2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Acid-sensing ion channel 1 (ASIC1) (Acid-sensing ion channel) (Amiloride-sensitive cation channel 2, neuronal) (Brain sodium channel 2) (BNaC2)

UniProt ID

Q6NXK8

Shipping

Shipped on ice packs

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Anti-ASIC1 Antibody (N271/44)

SKU: 75-277

Product Details

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