Apo Active FITC - Caspase 3 Detection Kit

This kit is highly specific for active human and murine enzyme. The kit utilizes a rabbit affinity purified polyclonal antibody against aa 163-175 of murine caspase 3. Visualization of fixed samples is via a FITC-labeled goat anti-rabbit antibody.



SKU: FAB200-2

Size: 100 Tests
Price:
Sale price$506

Apoptosis is an evolutionarily conserved form of cell suicide, which follows a specialized cellular process. The central component of this process is a cascade of proteolytic enzymes called caspases. These enzymes participate in a series of reactions that are triggered in response to pro-apoptotic signals and result in cleavage of protein substrates, causing the disassembly of the cell. Caspases have been identified in organisms ranging from C. elegans to humans. The mammalian caspases play distinct roles in apoptosis and inflammation. In apoptosis, caspases are responsible for proteolytic cleavages that lead to cell disassembly (effector caspases), and are involved in upstream regulatory events (initiator caspases). An active caspase consists of two large (~20 kD) and two small (~10 kD) subunits to form two heterodimers which associate in a tetramer. As is common with other proteases, caspases are synthesized as precursors that undergo proteolytic maturation, either autocatalytically or in a cascade by enzymes with similar specificity.

Caspase 3, also known as CPP-32, Apopain or Yama, is a key effector caspase in the apoptotic pathway. It is present in many different cell lineages and is responsible for the cleavage of a variety of molecules such as poly ADP-ribose polymerase (PARP), protein kinase C, actin and DNA-dependent protein kinase.

This kit utilizes a primary Rabbit affinity-purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3. This neo-epitope is present on the p18 subunit of cleaved caspase 3. Cell undergoing apoptosis are fixed and permeabilized prior to the addition of the primary antibody. A secondary FITC-labeled Goat anti-Rabbit antibody is used to visualize the bound Rabbit anti-caspase 3 polyclonal antibody.

Caspase-3/7
Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
2-8°C
Ships overnight (domestic), International Priority Shipping
United States
  • Part # 1002: Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial (2-8°C)
  • Part # 2002: FITC Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial (2-8°C)
  • Part # 3001: 10X Fixative, 1 Vial (2-8°C)
  • Product Specific References

    PMID Publication
    30529770Guo, Z., et al. 2019. Dendrobium candidum extract inhibits proliferation and induces apoptosis of liver cancer cells by inactivating Wnt/β-catenin signaling pathway. Biomedicine & Pharmacotherapy, 371-379.
    29435074Liu, J., et al. 2018. Over-activated PD-1/PD-L1 axis facilitates the chemoresistance of diffuse large B-cell lymphoma cells to the CHOP regimen. Oncology Letters, 3321-3328.

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