Our Anti-Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of mouse heart lysate showing specific immunolabeling of the ~250 kDa and ~150 kDa splice variants of the XIRP1 protein phosphorylated at Ser295 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295)
XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser-295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser-295 residue of XIRP1. (Chang et al., 2013).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
XIRP1
150, 250 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of mouse XIRP, conjugated to keyhole limpet hemocyanin (KLH).
Mouse
Canine, Human, Non-Human Primate, Rat
AB_2492287
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~250 kDa and ~150 kDa splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser295
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Chang, Y.W., et al. 2013. Quantitative phosphoproteomic study of pressure-overloaded mouse heart reveals dynamin-related protein 1 as a modulator of cardiac hypertrophy. Molecular & Cellular Proteomics, 12(11), 3094-3107.
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