Anti-WAVE1 (N-terminal region) Antibody

Name: Anti-WAVE1 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: WP1731
Price: 340.0 USD
Availability: InStock

1 Citation

Our WAVE1 (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat WAVE1 (N-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).

Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: WP1731

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

WAVE1 (N-terminal region)

Scientific Background

The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVE1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

WASF1

Molecular Weight

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal half of human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in human WAVE2 and WAVE3.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects an 80 kDa* protein corresponding to the molecular mass of WAVE1 on SDS-PAGE immunoblots of human SYF cSrc-transformed, HUVEC, and K562 cells, as well as rat PC12 cells and mouse C2C12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Wiskott-Aldrich syndrome verproline, Scar1, WASF1

UniProt ID

Q92558

Shipping

Blue Ice

Product Specific References for Applications and Species

Immuno-Electron Microscopy: Mouse
Immunohistochemistry: Mouse
Western Blot: Mouse

Immuno-Electron Microscopy: Mouse
PMID	Dilution	Publication
31474830	1:100	Roesler, MK, et al. 2019. Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Frontiers in cellular neuroscience, 330.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
31474830	1:100	Roesler, MK, et al. 2019. Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Frontiers in cellular neuroscience, 330.

Western Blot: Mouse
PMID	Dilution	Publication
31474830	1:1000	Roesler, MK, et al. 2019. Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Frontiers in cellular neuroscience, 330.

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Anti-WAVE1 (N-terminal region) Antibody

SKU: WP1731

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-WAVE1 (N-terminal region) Antibody

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