Anti-WASH (C-terminal region) Antibody

Name: Anti-WASH (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: WP4001
Price: 348.0 USD
Availability: InStock

Our WASH rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat WASH and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of WASH relative to F-actin in chick DRG neurons. The cells were labeled with rabbit polyclonal WASH (C-terminal region) antibody (WP4001), then the antibody was detected using appropriate secondary antibody (Green). On the left, this WASH labeling is compared to F-actin staining (Red). (Image provided by Dr. Gianluca Gallo at Drexel University).

Western blot of human Jurkat cells (lanes 1-3). The blots were probed with anti-WASH (C-terminal region) rabbit polyclonal antibody at 1:250 (lane 1) or at 1:1000 in the absence (lane 2) or presence of WASH blocking peptide (WX4005) (lane 3).

Immunocytochemical labeling of WASH relative to F-actin in chick DRG neurons. The cells were labeled with rabbit polyclonal WASH (C-terminal region) antibody (WP4001), then the antibody was detected using appropriate secondary antibody (Green). On the left, this WASH labeling is compared to F-actin staining (Red). (Image provided by Dr. Gianluca Gallo at Drexel University).

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: WP4001

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

WASH

Scientific Background

The Wiskott–Aldrich syndrome protein (WASP) family functions downstream of Rho GTPases to mediate the branched-actin network formation required for cytoskeletal remodeling, intracellular transport, and cell motility. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms (WAVE1, 2, and 3). Two new WASP subfamilies are WASH and WHAMM. WASH is an F-actin binding protein that can promote Arp2/3-mediated branch actin nucleation. The WASH protein contains two WASH homology domains, a proline-rich region, and a C-terminal VCA domain. WASH can interact with dynamin, FAM21, and tubulin, and WASH activity may be regulated downstream of Rho GTPases. WASH interaction with FAM21 has been implicated in endosome sorting, and inactivation of WASH leads to endosome tubulation. Thus, WASH may be a critical element in the regulation of the actin filament and microtubule networks that mediate endosomal sorting and fission.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

WASHC1

Molecular Weight

70 kDa

Immunogen

WASH synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human WASH. This sequence is highly conserved in rat, mouse, and chicken WASH, and has low homology to other WASP family members.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_10616809

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Specificity

The antibody detects a 70 kDa* protein corresponding to the molecular mass of WASH on SDS-PAGE immunoblots of human Jurkat cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

FAM39E; WASH1; WASH complex subunit 1; CXYorf1-like protein on chromosome 9; FLJ00075 protein; WAS protein family homolog 1; actin nucleation promoting factor; family with sequence similarity 39; member E

UniProt ID

A8K0Z3
UniProt Summary: Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity.

NCBI Gene ID

100287171

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Anti-WASH (C-terminal region) Antibody

SKU: WP4001

Product Details

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