Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe..
phosphatase with tensin homology (voltage-sensing phosphoinositide phosphatase) ( VSP)
TPTEs (transmembrane phosphatases with tensin homology) aka VSPs (voltage sensing phosphatases) catalyze the dephosphorylation of phosphatidylinositol phosphates (PIPs) that regulate diverse aspects of cell membrane physiology including cell division, growth and migration (Ratzan W. et al., 2019). TPTEs are also thought to be involved in signal transduction pathways of the endocrine or spermatogenetic function of the testis (Chen H. et al., 1999).
Fusion protein amino acids 153-335 of zebrafish TPTE (also known as Transmembrane phosphatase with tensin homology, voltage-sensing phosphoinositide phosphatase and VSP, accession number Q4V9E4)
Chicken, Xenopus, Zebrafish
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